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Developmental Biology

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JoVE Journal Developmental Biology
A Guide to Examining Intramuscular Fat Formation and its Cellular Origin in Skeletal Muscle
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A Guide to Examining Intramuscular Fat Formation and its Cellular Origin in Skeletal Muscle

Article DOI: 10.3791/63996-v • 09:19 min • May 26th, 2022
May 26th, 2022 •
1, 1, 1,2
1Department of Pharmacology and Therapeutics, University of Florida College of Medicine, 2Myology Institute, University of Florida College of Medicine

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The replacement of healthy muscle tissue with intramuscular fat is a prominent feature of human diseases and conditions. This protocol outlines how to visualize, image, and quantify intramuscular fat, allowing the rigorous study of the mechanisms underlying intramuscular fat formation.

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Intramuscular Fat Skeletal Muscle Sarcopenia Neuromuscular Diseases Adipocytes Frozen Tissue Sections Adipocyte Morphology Visualization Quantification Interventions Duchenne Muscular Dystrophy Research Technician Alcohol Wipe Glycerol Injection TA Muscle Needle Insertion Mouse Dissection
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