Optimization of Transesophageal Atrial Pacing to Assess Atrial Fibrillation Susceptibility in Mice

Matthew B. Murphy; Kyungsoo Kim; Prince J. Kannankeril; Katherine T. Murray

doi:10.3791/64168

Optimización de la estimulación auricular transesofágica para evaluar la susceptibilidad a la fib...

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Optimization of Transesophageal Atrial Pacing to Assess Atrial Fibrillation Susceptibility in Mice

Optimización de la estimulación auricular transesofágica para evaluar la susceptibilidad a la fibrilación auricular en ratones

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08:05 min

June 29, 2022

DOI: 10.3791/64168-v

Matthew B. Murphy¹, Kyungsoo Kim¹, Prince J. Kannankeril¹, Katherine T. Murray¹

¹Departments of Medicine, Pediatrics and Pharmacology,Vanderbilt University School of Medicine

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Overview

This protocol describes the optimization of transesophageal atrial pacing to evaluate atrial fibrillation susceptibility in mice. The method allows for repeated testing in the same animal, enhancing the reproducibility of results.

Key Study Components

Research Area

Atrial fibrillation
Electrophysiology
Mouse models

Background

Understanding atrial fibrillation's electrophysiologic properties.
The importance of protocol standardization for experimental reproducibility.
Effects of pacing-induced parasympathetic stimulation on arrhythmias.

Methods Used

Transesophageal atrial pacing
Mice
ECG recording and analysis

Main Results

Optimal settings for pacing were established.
Data showed variations in AF susceptibility across different testing conditions.
Identified factors that could confound results, such as parasympathetic stimulation.

Conclusions

The study presents a refined protocol for assessing atrial fibrillation in mice.
It underlines the significance of meticulous protocol design in electrophysiological research.

Frequently Asked Questions

What is transesophageal atrial pacing?

It is a technique used to induce and study atrial fibrillation in experimental models, particularly in mice.

Why is it essential to optimize experimental parameters?

Optimizing parameters enhances the accuracy and reproducibility of research findings related to atrial fibrillation.

How can parasympathetic stimulation affect results?

It can lead to inadvertent induction of arrhythmias, confounding the interpretation of data regarding atrial fibrillation susceptibility.

What age should mice be for optimal testing?

Mice should be at least 12 weeks old for acquired models and start biweekly pacing at 8 weeks for genetic models.

What measures are taken to analyze atrial fibrillation?

Electrophysiologic parameters and duration of AF episodes are recorded, allowing for comprehensive analysis of susceptibility.

Why is serial testing important?

It allows researchers to assess changes in AF susceptibility over time in the same animal, improving data integrity.

Is this method applicable to genetic studies?

Yes, it is specifically designed to assess both genetic and acquired models of atrial fibrillation.

El presente protocolo describe la optimización de los parámetros experimentales cuando se utiliza la estimulación auricular transesofágica para evaluar la susceptibilidad a la fibrilación auricular en ratones.

La estimulación eléctrica programada mediante estimulación auricular transesofágica puede determinar las propiedades electrofisiológicas y la susceptibilidad a la fibrilación auricular en ratones. Aquí describimos un procedimiento estandarizado para el desarrollo de protocolos que puede optimizar la reproducibilidad. El enfoque transesofágico para la estimulación auricular es típicamente un procedimiento de supervivencia, lo que permite realizar pruebas seriadas en el mismo animal.

Los componentes importantes para el desarrollo del protocolo incluyen estudios iniciales de palets para optimizar los parámetros experimentales para el modelo en estudio y la identificación de arritmias causadas por la estimulación parasimpática inducida por la estimulación. Para los modelos genéticos, someten a ratones a estimulación auricular quincenal, comenzando a las ocho semanas de edad para determinar el período óptimo de susceptibilidad a la FA, estudian ambos sexos, ya que uno puede no desarrollar un fenotipo de FA. Para los modelos adquiridos, realice el ritmo después de que los ratones alcanzaron la madurez física.

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