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Biochemistry

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JoVE Journal Biochemistry
Hurtig vurdering af membranproteinkvalitet ved fluorescerende størrelseseksklusionskromatografi
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Hurtig vurdering af membranproteinkvalitet ved fluorescerende størrelseseksklusionskromatografi

Article DOI: 10.3791/64322-v • 06:26 min • January 6th, 2023
January 6th, 2023 •
1, 1, 1, 1
1Peak Proteins Ltd.

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Denne protokol beskriver en procedure til udførelse af fluorescerende størrelseseksklusionskromatografi (FSEC) på membranproteiner for at vurdere deres kvalitet til downstream funktionel og strukturel analyse. Repræsentative FSEC-resultater indsamlet for flere G-proteinkoblede receptorer (GPCR'er) under vaskemiddelopløselige og vaskemiddelfrie forhold præsenteres.

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Biokemi nr. 191
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