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Sperm Collection and Computer-Assisted Sperm Analysis in the Teleost Model Japanese Medaka (Oryzias latipes)

Article DOI: 10.3791/64326-v • 06:49 min • October 6th, 2022
October 6th, 2022 •
1, 2, 1
1Department of Preclinical Sciences and Pathology, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, 2Department of Production Animal Clinical Sciences, Faculty of Veterinary Medicine, Norwegian University of Life Sciences

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This article describes two quick and efficient methods for collecting sperm from the small model fish medaka (Oryzias latipes), as well as a protocol for reliably assessing sperm quality using computer-assisted sperm analysis (CASA).

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Sperm Collection Computer-assisted Sperm Analysis Teleost Model Japanese Medaka Oryzias Latipes Milt Sampling Sperm Quality Abdominal Massage Repeated Sampling Non-invasive Approach Cryo Preservation Fertilization Success Ecological Studies Productive Physiology Studies Soft Holding Sponge Anesthetizing The Fish Drying The Abdomen Micro Pipette Aspirator Tube
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