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Biochemistry

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JoVE Journal Biochemistry
Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles
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Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

Article DOI: 10.3791/64360-v • 10:16 min • January 20th, 2023
January 20th, 2023 •
1, 2, 3, 3, 3, 3, 3, 2
1Central Laboratory of Women’s Hospital, Zhejiang University School of Medicine, 2School of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, 3Core Facilities, Zhejiang University School of Medicine

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This protocol provides a rapid and size-specific isolation method for small extracellular vesicles by optimizing the size of the air spray nozzle, sheath fluid pressure, sample flow pressure, voltage, gain, and triggering threshold parameters.

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Flow Cytometric Sorting High-throughput Isolation Purification Small Extracellular Vesicles Optimization Sorting Parameters Flow Cytometry Forward Scatter Size Scatter Startup Procedure Laser Power Smart Sampler Submenu Pressure Console Sheath Fluid Sample Flow Sheath Stream Bubble Removal Alignment Laser Spot
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