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An Enrichment Method for Small Extracellular Vesicles Derived from Liver Cancer Tissue
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Investigación sobre el cáncer
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JoVE Revista Investigación sobre el cáncer
An Enrichment Method for Small Extracellular Vesicles Derived from Liver Cancer Tissue

An Enrichment Method for Small Extracellular Vesicles Derived from Liver Cancer Tissue

DOI:
10.3791/64499-v

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10:33 min

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February 03, 2023

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, , , , , ,
1The First School of Clinical Medicine,Gannan Medical University, 2Laboratory Medicine,First Affiliated Hospital of Gannan Medical University

Capítulos

  • 00:04Introduction
  • 00:46Tissue Dissociation
  • 02:13Differential Ultracentrifugation
  • 04:42Evaluation of Enrichment Quality
  • 09:04Results: Small Extracellular Vesicles Characterization and Analysis
  • 10:02Conclusion

Summary

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Traducción Automática

Here we describe the enrichment of small extracellular vesicles derived from liver cancer tissue through an optimized differential ultracentrifugation method.

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Small Extracellular VesiclesEnrichment MethodDifferential UltracentrifugationSEV IsolationPBS WashingSEV Resuspension

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