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Immunology and Infection

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Quantifying Myeloperoxidase-DNA and Neutrophil Elastase-DNA Complexes from Neutrophil Extracellular Traps by Using a Modified Sandwich ELISA

Article DOI: 10.3791/64644-v • 07:18 min • May 12th, 2023
May 12th, 2023 •
1, 2, 2, 2, 2
1Department of Biochemistry and Biotechnology, University of Science and Technology Chittagong (USTC), 2Department of Emergency and Critical Care Medicine, Aichi Medical University

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We present a protocol for a modified sandwich enzyme-linked immunosorbent assay technique to quantitatively measure two components of neutrophil extracellular trap remnants, myeloperoxidase conjugated-DNA and neutrophil elastase conjugated-DNA complexes,derived from activated neutrophils.

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Myeloperoxidase-DNA Neutrophil Elastase-DNA Neutrophil Extracellular Traps (NETs) Sandwich ELISA Clinical Settings Sepsis Septic ARDS Acute Respiratory Distress Syndrome Dumps Or Damage-associated Molecular Patterns Circulating NET Remnants Incubation Times Polymorphonuclear Neutrophils (PMNs) Phenol Red-free RPMI 1640 PMA Stimulation DNase I Digestion EDTA
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