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Concanavalin A-Based Sedimentation Assay to Measure Substrate Binding of Glucan Phosphatases

Article DOI: 10.3791/64700-v • 09:07 min • December 23rd, 2022
December 23rd, 2022 •
*1, *1, 1, 1, 1
1Department of Chemistry, Skidmore College
* These authors contributed equally

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This method describes a lectin-based in vitro sedimentation assay to quantify the binding affinity of glucan phosphatase and amylopectin. This co-sedimentation assay is reliable for measuring glucan phosphatase substrate binding and can be applied to various solubilized glucan substrates.

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Concanavalin A-based Sedimentation Assay Substrate Binding Glucan Phosphatases Binding Affinity STA Substrates Lectin-based Cosedimentation Assay Millimolar Range Characterization Of SEX4 Mutants Protein Carbohydrate Interactions Concentration Range ConA-sepharose Beads Suspension Centrifugation Binding Buffer Solubilized Amylopectin Solution
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