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Human Mesenchymal Stem Cell Processing for Clinical Applications Using a Closed Semi-Automated Workflow
JoVE Revista
Bioquímica
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JoVE Revista Bioquímica
Human Mesenchymal Stem Cell Processing for Clinical Applications Using a Closed Semi-Automated Workflow

Human Mesenchymal Stem Cell Processing for Clinical Applications Using a Closed Semi-Automated Workflow

DOI:
10.3791/64707-v

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09:03 min

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March 17, 2023

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, , , , , ,
1Bioprocessing Technology Institute (BTI),Agency for Science, Technology and Research (A STAR), 2Thermo Fisher Scientific, 3Thermo Fisher Scientific

Capítulos

  • 00:04Introduction
  • 01:01Closed Semi‐Automated WJ‐hMSC Dissociation and Harvesting Using Closed Counterflow Centrifugation
  • 03:56Running the Protocol
  • 06:03Results: Characterizing the WJ‐MSCs Harvested Using the Closed Semi‐Automated Counterflow Centrifugation
  • 08:15Conclusion

Summary

Traducción Automática

Traducción Automática

Here, we present a protocol to harvest adherent cells from multi-layered flasks in a closed semi-automated manner using a counterflow centrifugation system. This protocol can be applied for harvesting both adherent and suspension cells from other cell expansion platforms with few modifications to the existing steps.

Tags

Mesenchymal Stem CellsMSCsWharton’s JellyClosed Automated Cell ProcessingCounterflow CentrifugationSingle-use KitCGMP ComplianceMulti-layered Culture SystemBenchtop CentrifugationBioprocessingCell HarvestingCell WashingCell Dissociation

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