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JoVE Journal Bioengineering
Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro
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Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro

Article DOI: 10.3791/64712-v • 11:52 min • April 21st, 2023
April 21st, 2023 •
1, 3, 1,2,3
1Microbiology Graduate Program, University of California, Riverside, 2Department of Bioengineering, University of California, Riverside, 3Materials Science & Engineering Program, University of California, Riverside

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We introduce four methods to evaluate the antimicrobial activities of nanoparticles and nanostructured surfaces using in vitro techniques. These methods can be adapted to study the interactions of different nanoparticles and nanostructured surfaces with a broad range of microbial species.

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Antimicrobial Activities Nanoparticles Nanostructured Surfaces In Vitro Protocols Preliminary Stage Investigations Surface Examinations Data Comparability Nanoparticle Materials Nanostructured Materials Microbial Species Homogenous Distribution Vortexing Samples Nanoparticle Suspension Pipetting Cultured Bacteria Overnight Grown Bacterial Culture Micro Centrifuge Tubes Supernatant Collection Cell Pellet Resuspension Growth Medium Centrifugalization Wash With Tris Buffer Or Hydroxyethyl Aminomethane Buffer
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