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A 3D Culture Method of Spheroids of Embryonic and Liver Zebrafish Cell Lines
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Medio ambiente
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JoVE Revista Medio ambiente
A 3D Culture Method of Spheroids of Embryonic and Liver Zebrafish Cell Lines

A 3D Culture Method of Spheroids of Embryonic and Liver Zebrafish Cell Lines

DOI:
10.3791/64859-v

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08:00 min

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January 20, 2023

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, , , , , , , , ,
1Graduate Program in Genetics, Department of Genetics,Federal University of Paraná (UFPR), 2Safety of Product Department,Grupo Boticário, 3Department of Cell Biology,Federal University of Paraná (UFPR), 4Department of Genetics,Federal University of Paraná (UFPR)

Capítulos

  • 00:05Introduction
  • 00:55Cell Culture Medium and Monolayer Cultures
  • 02:20Cell Counting with Trypan Blue Dye Exclusion Test
  • 03:07Cell Plating in ULA-Plates
  • 04:48Results: Characterization of Spheroids Developed with 3D Spheroid Method
  • 07:12Conclusion

Summary

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Traducción Automática

Here, we present an effective, easy, and fast 3D culture protocol for the formation of spheroids of two zebrafish (Danio rerio) cell lines: ZEM2S (embryo) and ZFL (normal hepatocyte).

Tags

3D Cell CultureZebrafish Cell LinesSpheroidsIn Vitro ModelToxicity TestingCell ViabilityTrypan BlueCell Counting96-well ULA Plate

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