Generation and Culturing of High-Grade Serous Ovarian Cancer Patient-Derived Organoids

Olivia Graham; Jeimmy Rodriguez; Lillian van Biljon; Bisiayo Fashemi; Emily Graham; Katherine Fuh; Dineo Khabele; Mary Mullen

doi:10.3791/64878

Generación y cultivo de organoides derivados de pacientes con cáncer de ovario seroso de alto grado

JoVE Journal
Cancer Research

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JoVE Journal Cancer Research

Generation and Culturing of High-Grade Serous Ovarian Cancer Patient-Derived Organoids

Generación y cultivo de organoides derivados de pacientes con cáncer de ovario seroso de alto grado

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07:19 min

January 6, 2023

DOI: 10.3791/64878-v

Olivia Graham*¹, Jeimmy Rodriguez*¹, Lillian van Biljon¹, Bisiayo Fashemi¹, Emily Graham¹, Katherine Fuh^1,2, Dineo Khabele¹, Mary Mullen¹

¹Washington University in St. Louis, ²University of California San Francisco

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Overview

Patient-derived organoids (PDO) are three-dimensional cultures that accurately mimic the tumor environment in vitro, particularly in high-grade serous ovarian cancer. These models are essential for studying novel biomarkers and therapeutics.

Key Study Components

Area of Science

Neuroscience
Oncology
Cell Biology

Background

PDOs represent a reliable model for studying ovarian cancer.
They recapitulate clonal heterogeneity and the tumor microenvironment.
PDOs overcome limitations of traditional cancer cell lines and patient-derived xenografts.
These organoids offer long-term expansion and mimic tumor physiology.

Purpose of Study

To study novel therapeutics in ovarian cancer.
To identify predictive biomarkers.
To understand complex biological processes in cancer.

Methods Used

Harvesting organoids from tumor samples.
Mincing tissue to create a homogenous mixture.
Using a dissociation tube for processing.
In vitro culture techniques for organoid maintenance.

Main Results

PDOs effectively mimic the tumor environment.
They allow for the study of therapeutic responses.
PDOs maintain genetic and functional characteristics of parent tumors.
They provide insights into tumor biology and treatment efficacy.

Conclusions

PDOs are ideal for studying ovarian cancer therapeutics.
They represent a significant advancement over traditional models.
These models can lead to better understanding and treatment of ovarian cancer.

Frequently Asked Questions

What are patient-derived organoids?

Patient-derived organoids are 3D cultures that mimic the tumor environment, allowing for the study of cancer biology and therapeutics.

How are organoids harvested?

Organoids are harvested by mincing tumor samples and processing them into a homogenous mixture for culture.

What advantages do PDOs have over traditional cancer models?

PDOs recapitulate tumor heterogeneity and microenvironment, providing a more accurate model for research.

Can PDOs be used for long-term studies?

Yes, PDOs offer long-term expansion and storage capabilities for ongoing research.

What types of cancer can PDOs be used to study?

PDOs can be used to study various cancers, including ovarian cancer, by mimicking the tumor physiology.

Are PDOs effective for testing new therapies?

Yes, PDOs are effective for testing novel therapeutics and understanding their impact on tumor biology.

Los organoides derivados del paciente (PDO) son un cultivo tridimensional (3D) que puede imitar el entorno tumoral in vitro. En el cáncer de ovario seroso de alto grado, las PDO representan un modelo para estudiar nuevos biomarcadores y terapias.

Los modelos de organoides impulsados por pacientes representan genética y funcionalmente sus tumores progenitores y, por lo tanto, son modelos confiables para estudiar nuevas terapias y procesos biológicos complejos en el cáncer de ovario. Al recapitular la heterogeneidad clonal, el microambiente tumoral y las interacciones in vitro de célula a célula, estos modelos superan las limitaciones de las líneas celulares cancerosas y los xenoinjertos derivados del paciente. Los organoides derivados del paciente ofrecen capacidades de expansión y almacenamiento a largo plazo e imitan la fisiología tumoral.

Como resultado, son modelos ideales para el estudio de nuevas terapias y biomarcadores predictivos. Comience la recolección de los organoides colocando las muestras de tumor en un plato de cultivo de tejido de 10 centímetros y picando el tejido para crear una mezcla homogénea usando un bisturí desechable. Coloque la mezcla de tejido homogéneo en un tubo de disociación con fórceps.

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