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Microvascular and Macrovascular Endothelial Cell Isolation and Purification from Lung-Derived Samples

Article DOI: 10.3791/64885-v • 05:26 min • February 3rd, 2023
February 3rd, 2023 •
1,2, 2, 2,3, 2,3, 1, 4, 4,5, 1,2, 2,3, 1, 6,7, 1, 1,2
1Department of Medical, Oral and Biotechnological Sciences, “G. d’Annunzio” University of Chieti-Pescara, 2Center on Advanced Studies and Technology (CAST), “G. d’Annunzio” University of Chieti-Pescara, 3Department of Medicine and Aging Sciences, University “G. d’Annunzio” of Chieti-Pescara, 4U.O. di Chirurgia Toracica e dei Trapianti di Polmone, Fondazione IRCCS Ca’ Granda - Ospedale Maggiore Policlinico di Milano, 5Dipartimento di Fisiopatologia Medico-Chirurgica e dei Trapianti, Università degli Studi di Milano, 6Department of Pharmaceutical Sciences, Università degli Studi di Milano, 7Unit of Cell and Molecular Biology in Cardiovascular Diseases, Centro Cardiologico Monzino IRCCS

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Although challenging, the isolation of pulmonary endothelial cells is essential for studies on lung inflammation. The present protocol describes a procedure for the high-yield, high-purity isolation of macrovascular and microvascular endothelial cells.

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Microvascular Endothelial Cell Isolation Macrovascular Endothelial Cell Isolation Purification Lung-derived Samples Tissue Samples Blood Vessels Technique Reproducibility Good Purity Lung Parenchyma Sample Washing PBS Without Calcium Chloride And Magnesium Chloride Sterile Petri Dish Small Fragments Surgical Scissors Artery Segment Residual Blood Removal PBS Minus Minus Washes Diced Lung Parenchyma Pulmonary Artery Segment Type II Collagenase Incubation One-millimeter Mesh-size Strainer 50-milliliter Collection Tube
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