Journal
/
Investigación sobre el cáncer
/
Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence
JoVE Revista
Investigación sobre el cáncer
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Investigación sobre el cáncer
Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence

Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence

DOI:
10.3791/64920-v

•

06:25 min

•

February 10, 2023

•

, ,
1Division of Oncology, Department of Medicine, Siteman Cancer Center,Washington University School of Medicine

Capítulos

  • 00:05Introduction
  • 01:00Plating, Pulsing, Fixation, Permeabilization, and Blocking of Cells
  • 03:59Immunostaining with the IdU Antibody
  • 05:15Results: IdU-Induced Replication Stress
  • 05:41Conclusion

Summary

Traducción Automática

Traducción Automática

Here, we describe an immunofluorescence-based method to quantify the levels of single-stranded DNA in cells. This efficient and reproducible method can be utilized to examine replication stress, a common feature in several ovarian cancers. Additionally, this assay is compatible with an automated analysis pipeline, which further increases its efficiency.

Tags

Replication StressSingle-stranded DNAImmunofluorescenceOvarian CancerBRCA1BRCA2Cell LinesBiomarkerDNA RepairHigh-throughputQuantificationProtocol

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

The Drosophila Imaginal Disc Tumor Model: Visualization and Quantification of Gene Expression and Tumor Invasiveness Using Genetic Mosaics

Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization

Evaluating In Vitro DNA Damage Using Comet Assay

Immunofluorescence Microscopy of γH2AX and 53BP1 for Analyzing the Formation and Repair of DNA Double-strand Breaks

Characterization of Tumor Cells Using a Medical Wire for Capturing Circulating Tumor Cells: A 3D Approach Based on Immunofluorescence and DNA FISH

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy

Semi-automatic PD-L1 Characterization and Enumeration of Circulating Tumor Cells from Non-small Cell Lung Cancer Patients by Immunofluorescence

Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells

Modeling the Effects of Hemodynamic Stress on Circulating Tumor Cells using a Syringe and Needle

Orthotopic Implantation of Patient-Derived Cancer Cells in Mice Recapitulates Advanced Colorectal Cancer

Read Article