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Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence
JoVE Revista
Investigación sobre el cáncer
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JoVE Revista Investigación sobre el cáncer
Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence
DOI:

06:25 min

February 10, 2023

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Capítulos

  • 00:05Introduction
  • 01:00Plating, Pulsing, Fixation, Permeabilization, and Blocking of Cells
  • 03:59Immunostaining with the IdU Antibody
  • 05:15Results: IdU-Induced Replication Stress
  • 05:41Conclusion

Summary

Traducción Automática

Here, we describe an immunofluorescence-based method to quantify the levels of single-stranded DNA in cells. This efficient and reproducible method can be utilized to examine replication stress, a common feature in several ovarian cancers. Additionally, this assay is compatible with an automated analysis pipeline, which further increases its efficiency.

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