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Isolation and Purification of Fungal β-Glucan as an Immunotherapy Strategy for Glioblastoma

Article DOI: 10.3791/64924-v • 10:02 min • June 2nd, 2023
June 2nd, 2023 •
*1, *1, 1, 1, 1,2, 1, 1,2, 1, 1,2
1Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, Universidad de Sevilla, 2Instituto de Biomedicina de Sevilla, Hospital Universitario Virgen del Rocío, CSIC, Universidad de Sevilla
* These authors contributed equally

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The present protocol describes the purification steps and subsequent studies of four different fungal β-glucans as potential immunomodulatory molecules that enhance the anti-tumoral properties of microglia against glioblastoma cells.

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Isolation Purification Fungal Beta-glucan Immunotherapy Strategy Glioblastoma Co-adjuvants Cancer Therapy Beta-glucans Microglia Phenotype Modulation Pleurotus Ostreatus Pleurotus Djamor Hericium Erinaceus Ganoderma Lucidum Soluble Mushroom Polysaccharides (SMPs) Fruiting Bodies Distilled Water Air Drying Oven Blade Mill Mushroom Powder Suspension Autoclave Incubation Centrifuge Insoluble Mushroom Polysaccharides (IMPs)
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