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Isolation and Characterization of the Murine Uterosacral Ligaments and Pelvic Floor Organs

Article DOI: 10.3791/65074-v • 05:47 min • March 3rd, 2023
March 3rd, 2023 •
1, 2, 2, 3, 2,5, 1,2,4, 1,2,4
1Biomedical Engineering Program, University of Colorado Boulder, 2Paul M. Rady Department of Mechanical Engineering, University of Colorado Boulder, 3Department of Obstetrics and Gynecology, University of Colorado Anschutz Medical Campus, 4BioFrontiers Institute, University of Colorado Boulder, 5Department of Mechanical Science and Engineering, University of Illinois Urbana-Champaign

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This article presents a detailed protocol for dissecting uterosacral ligaments and other pelvic floor tissues, including the cervix, rectum, and bladder in mice, to expand the study of female reproductive tissues.

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Isolation Characterization Murine Uterosacral Ligaments Pelvic Floor Organs Pelvic Organ Prolapse POP Dissection Mirroring Pelvic Floor Tissue Preclinical Model Pregnancy Disease Tissue Mechanical Behavior Women's Health-related Research Identifying Handling Uterosacral Ligament Euthanized Mouse Incision Abdomen Scissors Skin Separation Dorsal Side Up Hind Limbs Thorax Pelvis Irrigation With PBS Forceps Pelvic Fat Tissue Uterine Horns
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