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DOI: 10.3791/65120-v
Haifeng Shao1,2,3,4, Jialin Deng2,3,4, Yanqing Liu1,2,3,4, Niuniu Yang1,2,3,4
1Department of Traditional Chinese Medicine, The Affiliated Hospital of Yangzhou University,Yangzhou University, 2Department of Traditional Chinese and Western Medicine, Medical College,Yangzhou University, 3The Key Laboratory of Syndrome Differentiation and Treatment of Gastric Cancer of the State Administration of Traditional Chinese Medicine,Yangzhou University, 4Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases,Yangzhou University
Please note that some of the translations on this page are AI generated. Click here for the English version.
Aquí, describimos los métodos para inducir dermatitis alérgica de contacto en orejas de ratón por 1-fluoro-2,4-dinitrobenceno (DNFB) y cómo evaluar la gravedad de la dermatitis alérgica de contacto.
Este modelo no solo se puede utilizar para explorar la patogénesis de la ACD, sino que también es aplicable para la evolución preclínica de nuevos métodos terapéuticos, que tiene un significado importante. Este es un modelo animal estable y eficiente con muchas ventajas, como un corto período experimental, fácil operación, síntomas obvios y una alta tasa de éxito. Nuestro protocolo experimental es muy detallado y fácil de operar.
Asegúrese de seguir cada paso del protocolo correctamente. Antes de comenzar el modelado, aclimatar a los ratones al medio ambiente durante una semana con acceso gratuito a alimentos y agua. El día del modelado, utilizando una lámpara ultravioleta y 75% de alcohol, limpie y desinfecte el ambiente y las encimeras.
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