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Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Semi-Automated Isolation of Murine White Adipose Tissue

Article DOI: 10.3791/65265-v • 02:39 min • May 19th, 2023
May 19th, 2023 •
1,2, 1,2,3, 2, 2
1Division for Health Service Promotion, The University of Tokyo, 2Department of Diabetes and Metabolic Diseases, Graduate School of Medicine, The University of Tokyo, 3The University of Tokyo Excellent Young Researcher Program, The University of Tokyo

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Semi-Automated Isolation Stromal Vascular Fraction Murine White Adipose Tissue Tissue Dissociator Brown Adipocyte Beige Adipocyte Energy Expenditure Obesity Treatment Diabetes Treatment NFI Transcription Factor Adipogenesis PPAR-gamma Brown-fat-specific Enhancers Collagenase Digestion Experimental Variability Contamination Reproducibility
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