Modified <em>In Vivo</em> Matrix Gel Plug Assay for Angiogenesis Studies

Zixian Lu; Mei Yi; Taoli Chen; Yue He; Xia Fan; Hui Chen; Yinli Huang; Jianlou Niu; Xiaoqing Yan

doi:10.3791/65567

Ensayo de tapón de gel de matriz in vivo modificado para estudios de angiogénesis

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Modified In Vivo Matrix Gel Plug Assay for Angiogenesis Studies

Ensayo de tapón de gel de matriz in vivo modificado para estudios de angiogénesis

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09:03 min

June 30, 2023

DOI: 10.3791/65567-v

Zixian Lu*^1,2, Mei Yi*², Taoli Chen*², Yue He*², Xia Fan*², Hui Chen², Yinli Huang¹, Jianlou Niu^1,2, Xiaoqing Yan^1,2

¹Pingyang Affiliated Hospital of Wenzhou Medical University, ²School of Pharmaceutical Sciences,Wenzhou Medical University

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Overview

This study investigates angiogenesis, the formation of new blood vessels, using a modified matrix gel plug assay. The method allows for the evaluation of the effects of reagents on angiogenesis and vascular permeability in vivo without staining.

Key Study Components

Research Area

Angiogenesis
Vascular permeability
In vivo methodologies

Background

Angiogenesis is crucial for various physiological and pathological processes.
Existing models for studying angiogenesis are often complex and expensive.
A simpler and efficient model is needed for evaluating angiogenesis and vessel permeability.

Methods Used

Modified matrix gel plug assay
Mouse model for in vivo studies
Dextran-FITC injection for visualization of neo-vessels

Main Results

The method effectively visualized neo-vessels and vascular leakage without the need for staining.
It demonstrated the feasibility of evaluating angiogenesis using a simple and intuitive approach.
The study validates the use of this method for future research.

Conclusions

This study presents a novel method for assessing angiogenesis and vascular permeability.
The method can significantly advance research in angiogenesis-dependent diseases.

Frequently Asked Questions

What are the advantages of the modified matrix gel plug assay?

The assay simplifies the evaluation process by eliminating the need for complex staining techniques.

How does dextran-FITC help in the study?

Dextran-FITC allows for clear visualization of new blood vessels and helps assess vascular permeability.

What implications does this research have for medical studies?

The method can facilitate drug discovery and development for diseases associated with abnormal angiogenesis.

Is this method applicable to other biological studies?

Yes, the assay can be adapted for various studies related to vascular biology.

Are there any specific conditions required for the cell culture?

Yes, the cells should be cultured in a specific endothelial medium at controlled temperatures and CO2 levels.

Can this method be used with different animal models?

While this study used mice, the methodology can potentially be adapted for other small animal models.

What types of reagents can be tested with this method?

The method is versatile and can be utilized to test various reagents or drugs affecting angiogenesis.

El método aquí presentado puede evaluar el efecto de los reactivos sobre la angiogénesis o la permeabilidad vascular in vivo sin tinción. El método utiliza la inyección de dextrano-FITC a través de la vena de la cola para visualizar los neovasos o la fuga vascular.

Nuestra investigación se centra en la angiogénesis, el proceso de formación de nuevos vasos a partir de vasos preexistentes. Desempeña un papel fundamental en muchos procesos fisiológicos y patológicos, como el desarrollo embrionario o la curación de la aterosclerosis, el desarrollo de tumores, etc. La construcción de un modelo fiable para evaluar la extensión de la angiogénesis es fundamental para la investigación miocárdica o el descubrimiento de medicamentos para las enfermedades dependientes de la angiogénesis.

Sin embargo, la mayoría de los modelos existentes son complejos, caros y requieren equipos especializados. Aquí, presentamos un ensayo de tapón de gel de matriz modificada que se puede utilizar para investigar la angiogénesis y la permeabilidad de los vasos sin tinción de una manera simple, más intuitiva y eficiente.

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