A Preterm Rat Model for Pain Studies

Ravena Carolina de Carvalho; Ana Laura Silva; Danielle Cavalcante de Paula; Laura Pereira Generoso; Natalie Lange Candido; Maria Gabriela Maziero Capello; Gabrielly Santos Pereira; Carlos Marcelo de Barros; Danielle Mazetto Cadide; Josie Resende Torres da Silva; Marcelo Louren&#231;o da Silva

doi:10.3791/65800

Un modelo de rata prematura para estudios del dolor

JoVE Journal
Neuroscience
Author Produced

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Neuroscience

A Preterm Rat Model for Pain Studies

Un modelo de rata prematura para estudios del dolor

Full Text

813 Views

01:37 min

February 9, 2024

DOI: 10.3791/65800-v

Ravena Carolina de Carvalho¹, Ana Laura Silva¹, Danielle Cavalcante de Paula¹, Laura Pereira Generoso¹, Natalie Lange Candido¹, Maria Gabriela Maziero Capello¹, Gabrielly Santos Pereira¹, Carlos Marcelo de Barros^1,2, Danielle Mazetto Cadide^1,2, Josie Resende Torres da Silva¹, Marcelo Lourenço da Silva¹

¹Laboratory of Neuroscience, Neuromodulation and Study of Pain (LANNED),UNIFAL-MG, ²SINPAIN Faculty

Please note that some of the translations on this page are AI generated. Click here for the English version.

Overview

This study presents a protocol for establishing a preterm rat model to aid in research focused on early postnatal pain management. The method includes a cesarean section performed three days before expected birth to extract preterm rat pups and facilitate their adoption by a surrogate dam's biological offspring.

Key Study Components

Area of Science

Neuroscience
Developmental Biology

Background

Understanding pain management in preterm neonates is crucial for improving neonatal care.
Creating a reliable animal model can provide insight into pain mechanisms and management strategies.
The protocol aims to enhance research capabilities involving pain in preterm subjects.

Purpose of Study

To develop a reproducible method for studying pain in preterm rats.
To facilitate the investigation of postnatal care interventions.

Methods Used

Perform a cesarean section to obtain preterm rat pups.
Utilize hysterectomy for pup extraction and manage the pups' early care.
Integrate preterm pups with biological offspring of a surrogate dam.
Key steps include cleaning airways, cord management, and ensuring adoption success.

Main Results

The established protocol effectively allows for the integration of preterm rat pups into surrogate litters.
Careful management of hygiene and early handling contributes to successful adoption.

Conclusions

The study demonstrates a significant advance in creating a preterm rat model for pain research.
Implications include improved understanding of interventions for neonatal pain management.

Frequently Asked Questions

What are the advantages of using a preterm rat model?

Preterm rat models allow researchers to study the effects of early interventions on pain management in neonates, providing insights applicable to clinical practices.

How is the preterm rat model established?

The model is created through a cesarean section performed three days preterm, followed by careful management of the extracted pups.

What types of outcomes can be studied with this model?

Researchers can investigate pain responses, effects of treatments, and behaviors associated with early postnatal care in preterm rats.

Can the protocol be adapted for other species?

While optimized for rats, the fundamental techniques may be adapted for similar studies in other mammalian species with appropriate modifications.

What are some key limitations of this method?

One limitation is the complexity involved in ensuring a successful adoption by the surrogate dam, which can vary between individual animals.

How does the procedure ensure the well-being of the preterm pups?

The procedure includes vital steps for cleaning, warming, and integrating pups into the surrogate nest to minimize stress and mortality.

Aquí, presentamos un protocolo conciso para crear un modelo de rata prematura, facilitando la investigación sobre el manejo temprano del dolor posnatal. El método consiste en realizar una cesárea tres días antes del parto previsto, extraer crías de rata prematuras mediante histerectomía e integrarlas con la descendencia biológica de una madre sustituta.

Para empezar, consigue una madre embarazada pretérmino parcialmente anestesiada. Después de la eutanasia, haga un corte de tres centímetros en la línea media de la parte inferior del abdomen, seguido de un corte longitudinal de dos centímetros. Luego, haga una incisión a lo largo del borde antimesentérico en la porción de la línea media de cada trompa uterina de estos cortes.

Luego realice una histerectomía y extraiga suavemente las crías de rata y la placenta una por una. Baña al cachorro para evitar el canibalismo mediante madres sustitutas. Después de secar al cachorro, elimine cualquier rastro de sangre.

View the full transcript and gain access to thousands of scientific videos

Explore More Videos

Palabras clave: Pretérmino Dolor Modelo de Rata Estimulación por Pinchazo Hipersensibilidad Mecánica Hipersensibilidad Inflamatoria Respuestas Nociceptivas Dolor Neonatal Procesamiento Sensorial Receptores de Glucocorticoides