So the focus of our laboratory is to understand the molecular basis of multiple sclerosis, a immune disease of the central nervous system that is characterized by demyelination, neuroinflammation, and axonal injury. So this protocol represent an economical and flexible alternatives to commercial kits. Our can be easily adjusted to test B-cell antibody responses against different EEA peptides.
My lab is devoted to further understanding the connection between neuroinflammation and neurodegeneration in chronic neurological diseases such as multiple sclerosis.
Carver, J. J., Didonna, A. Quantification of Autoreactive Antibodies in Mice upon Experimental Autoimmune Encephalomyelitis. J. Vis. Exp. (202), e66218, doi:10.3791/66218 (2023).