Procedimientos de Operación y Biocontención de una Instalación para Ratones de Laboratorio con un Microbioma Natural: Procedimiento de Inmunofenotipado
1.4K visualizaciones
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05:34 min.
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13 de diciembre de 2024
DOI :10.3791/67100-v
13 de diciembre de 2024
•1.4K visualizaciones
Natascha Drude1 , Kai Diederich2 , Claudia U. Duerr3 , Nadine Haase4 , Christoph Harms5,6,7,8 , Frank Heppner9,10,11 , Marina Jendrach9,10,11 , Pia Kahnau2 , Marina Kolesnichenko12 , Lars Lewejohann2,13 , Claudia Kurreck1 , Anke Lohan14 , Marcus A. Mall15,16 , Dominik Müller4 , Stefan Nagel-Riedasch14 , Bastian Opitz17 , Laura Schaupp15,16 , Gilbert Schönfelder2,18 , Amelie Weber3 , Gerald Willimsky19,20,21 , Yalei Zang1,22 , Stephan P. Rosshart*23,24 , Andreas Diefenbach*3 , Stefan Jordan*3
1BIH QUEST Center for Responsible Research, Berlin Institute of Health (BIH) at Charité - Universitätsmedizin Berlin, 2German Federal Institute for Risk Assessment (BfR), German Centre for the Protection of Laboratory Animals (Bf3R), 3Institute of Microbiology, Infectious Diseases and Immunology, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 4Experimental and Clinical Research Center (ECRC) & Max-Delbrück-Centrum für Molekulare Medizin in der Helmholtz-Gemeinschaft (MDC), 5Department of Experimental Neurology, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Klinik und Hochschulambulanz für Neurologie, 6Center for Stroke Research Berlin, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 7Einstein Center for Neuroscience, 8German Center for Cardiovascular Research (DZHK), partner site Berlin, 9Department of Neuropathology, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 10Cluster of Excellence, NeuroCure, 11German Center for Neurodegenerative Diseases (DZNE) Berlin, 12Department of Hepatology and Gastroenterology, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 13Institute of Animal Welfare, Animal Behavior and Laboratory Animal Science, Freie Universität Berlin, 14Forschungseinrichtungen für Experimentelle Medizin/FEM, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 15Department of Pediatric Respiratory Medicine, Immunology and Critical Care Medicine, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 16German Center for Lung Research (DZL), associated partner site, Berlin, 17Department of Infectious Diseases, Respiratory Medicine and Critical Care, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 18Division of Toxicology, Institute of Clinical Pharmacology and Toxicology, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 19Institute of Immunology, Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 20German Cancer Research Center, 21German Cancer Consortium, partner site Berlin, 22Department of Radiology (including Pediatric Radiology), Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 23Department of Microbiome Research, University Hospital Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), 24Department of Medicine II, Faculty of Medicine, Medical Center - University of Freiburg
* These authors contributed equally
Aquí, describimos la estructura y los procedimientos operativos, incluidas las medidas de contención microbiana de una instalación para "ratones salvajes" utilizando como ejemplo la toma de muestras de sangre para el inmunofenotipado.
Chapters in this video
0:00
Introduction
2:12
Sampling Blood from Wildling Mice Maintained with Natural Microbiomes
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