Name: Análisis por citometría de masas de las respuestas inmunitarias sistémicas y locales en el carcinoma hepatocelular (Video) | JoVE Journal
Uploaded: 2025-04-25T13:00:00.000+00:00
Duration: 8 min 25 s
Description: Aprende a analizar respuestas inmunitarias sistémicas y locales en carcinoma hepatocelular mediante citometría de masas. Este protocolo identifica 14 tipos celulares distintos en muestras de PBMC para estudiar mecanismos de evasión tumoral.

ai_agent_IP

ai_agent

maintenance

instantTrialDurationMinutes

isBiopharma

enableNewMyJoveSectionDesign

educationFeatures

seoMetaApi

ai_copilot_institution_blacklist

ai_chat_institution_blacklist

freeAccessWindow

ssoRoleCapture

scheduledMaintenanceBanner

joveLabsFeatures

disable_autoplay_for_bots

extended_trial_duration

5 minutes

<ol><li>Zhang, C. H., Cheng, Y., Zhang, S., Fan, J., Gao, Q. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Changing+epidemiology+of+hepatocellular+carcinoma+in+Asia%5BTitle%5D)+AND+%22Liver+Int%22%5BJournal%5D)">Changing epidemiology of hepatocellular carcinoma in Asia.</a> Liver Int. 42 (9), 2029-2041 (2022).</li>
<li>Renne, S. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Hepatocellular+carcinoma%3A+A+clinical+and+pathological+overview%5BTitle%5D)+AND+%22Pathologica%22%5BJournal%5D)">Hepatocellular carcinoma: A clinical and pathological overview.</a> Pathologica. 113 (3), 203-217 (2021).</li>
<li>Sayiner, M., Golabi, P., Younossi, Z. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Disease+burden+of+hepatocellular+carcinoma%3A+A+global+perspective%5BTitle%5D)+AND+%22Dig+Dis+Sci%22%5BJournal%5D)">Disease burden of hepatocellular carcinoma: A global perspective.</a> Dig Dis Sci. 64 (4), 910-917 (2019).</li>
<li>Zhang, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Risk+factors+and+prevention+of+viral+hepatitis-related+hepatocellular+carcinoma%5BTitle%5D)+AND+%22Front+Oncol%22%5BJournal%5D)">Risk factors and prevention of viral hepatitis-related hepatocellular carcinoma.</a> Front Oncol. 11, 686962(2021).</li>
<li>Zou, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Economic+burden+and+quality+of+life+of+hepatocellular+carcinoma+in+greater+China%3A+A+systematic+review%5BTitle%5D)+AND+%22Front+Public+Health%22%5BJournal%5D)">Economic burden and quality of life of hepatocellular carcinoma in greater China: A systematic review.</a> Front Public Health. 10, 801981(2022).</li>
<li>Li, Y., You, Z., Tang, R., Ma, X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Tissue-resident+memory+t+cells+in+chronic+liver+diseases%3A+Phenotype%2C+development+and+function%5BTitle%5D)+AND+%22Front+Immunol%22%5BJournal%5D)">Tissue-resident memory t cells in chronic liver diseases: Phenotype, development and function.</a> Front Immunol. 13, 967055(2022).</li>
<li>Shen, K. Y., Zhu, Y., Xie, S. Z., Qin, L. X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Immunosuppressive+tumor+microenvironment+and+immunotherapy+of+hepatocellular+carcinoma%3A+Current+status+and+prospectives%5BTitle%5D)+AND+%22J+Hematol+Oncol%22%5BJournal%5D)">Immunosuppressive tumor microenvironment and immunotherapy of hepatocellular carcinoma: Current status and prospectives.</a> J Hematol Oncol. 17 (1), 25(2024).</li>
<li>Oura, K., Morishita, A., Tani, J., Masaki, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Tumor+immune+microenvironment+and+immunosuppressive+therapy+in+hepatocellular+carcinoma%3A+A+review%5BTitle%5D)+AND+%22Int+J+Mol+Sci%22%5BJournal%5D)">Tumor immune microenvironment and immunosuppressive therapy in hepatocellular carcinoma: A review.</a> Int J Mol Sci. 22 (11), 5801(2021).</li>
<li>Chen, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Effect+of+infiltrating+immune+cells+in+tumor+microenvironment+on+metastasis+of+hepatocellular+carcinoma%5BTitle%5D)+AND+%22Cell+Oncol%22%5BJournal%5D)">Effect of infiltrating immune cells in tumor microenvironment on metastasis of hepatocellular carcinoma.</a> Cell Oncol. 46 (6), 1595-1604 (2023).</li>
<li>Du, Q., An, Q., Zhang, J., Liu, C., Hu, Q. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Unravelling+immune+microenvironment+features+underlying+tumor+progression+in+the+single-cell+era%5BTitle%5D)+AND+%22Cancer+Cell+Int%22%5BJournal%5D)">Unravelling immune microenvironment features underlying tumor progression in the single-cell era.</a> Cancer Cell Int. 24 (1), 143(2024).</li>
<li>Xu, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Reshaping+the+systemic+tumor+immune+environment+%28stie%29+and+tumor+immune+microenvironment+%28time%29+to+enhance+immunotherapy+efficacy+in+solid+tumors%5BTitle%5D)+AND+%22J+Hematol+Oncol%22%5BJournal%5D)">Reshaping the systemic tumor immune environment (stie) and tumor immune microenvironment (time) to enhance immunotherapy efficacy in solid tumors.</a> J Hematol Oncol. 15 (1), 87(2022).</li>
<li>Mao, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Crosstalk+between+cancer-associated+fibroblasts+and+immune+cells+in+the+tumor+microenvironment%3A+New+findings+and+future+perspectives%5BTitle%5D)+AND+%22Mol+Cancer%22%5BJournal%5D)">Crosstalk between cancer-associated fibroblasts and immune cells in the tumor microenvironment: New findings and future perspectives.</a> Mol Cancer. 20 (1), 131(2021).</li>
<li>Roederer, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Spectral+compensation+for+flow+cytometry%3A+Visualization+artifacts%2C+limitations%2C+and+caveats%5BTitle%5D)+AND+%22Cytometry%22%5BJournal%5D)">Spectral compensation for flow cytometry: Visualization artifacts, limitations, and caveats.</a> Cytometry. 45 (3), 194-205 (2001).</li>
<li>Sahaf, B., Rahman, A., Maecker, H. T., Bendall, S. C. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aSahaf%2C+B+%22Biomarkers+for+immunotherapy+of+cancer%3A+Methods+and+protocols%22">Biomarkers for immunotherapy of cancer: Methods and protocols</a>. , Springer. New York, New York, NY. (2020).</li>
<li>Turaç, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Combined+flow+cytometric+analysis+of+surface+and+intracellular+antigens+reveals+surface+molecule+markers+of+human+neuropoiesis%5BTitle%5D)+AND+%22PLoS+One%22%5BJournal%5D)">Combined flow cytometric analysis of surface and intracellular antigens reveals surface molecule markers of human neuropoiesis.</a> PLoS One. 8 (6), e68519(2013).</li>
<li>Wu, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Redefining+tumor-associated+macrophage+subpopulations+and+functions+in+the+tumor+microenvironment%5BTitle%5D)+AND+%22Front+Immunol%22%5BJournal%5D)">Redefining tumor-associated macrophage subpopulations and functions in the tumor microenvironment.</a> Front Immunol. 11, 1731(2020).</li>
<li>Elaldi, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((High+dimensional+imaging+mass+cytometry+panel+to+visualize+the+tumor+immune+microenvironment+contexture%5BTitle%5D)+AND+%22Front+Immunol%22%5BJournal%5D)">High dimensional imaging mass cytometry panel to visualize the tumor immune microenvironment contexture.</a> Front Immunol. 12, 666233(2021).</li>
<li>Gadalla, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Validation+of+cytof+against+flow+cytometry+for+immunological+studies+and+monitoring+of+human+cancer+clinical+trials%5BTitle%5D)+AND+%22Front+Oncol%22%5BJournal%5D)">Validation of cytof against flow cytometry for immunological studies and monitoring of human cancer clinical trials.</a> Front Oncol. 9, 415(2019).</li>
<li>Ijsselsteijn, M. E., Van Der Breggen, R., Farina Sarasqueta, A., Koning, F., De Miranda, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+40-marker+panel+for+high+dimensional+characterization+of+cancer+immune+microenvironments+by+imaging+mass+cytometry%5BTitle%5D)+AND+%22Front+Immunol%22%5BJournal%5D)">A 40-marker panel for high dimensional characterization of cancer immune microenvironments by imaging mass cytometry.</a> Front Immunol. 10, 2534(2019).</li>
<li>Zhang, Q., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Mass+cytometry-based+peripheral+blood+analysis+as+a+novel+tool+for+early+detection+of+solid+tumours%3A+A+multicentre+study%5BTitle%5D)+AND+%22Gut%22%5BJournal%5D)">Mass cytometry-based peripheral blood analysis as a novel tool for early detection of solid tumours: A multicentre study.</a> Gut. 72 (5), 996-1006 (2023).</li>
<li>Zheng, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Trajectory+and+functional+analysis+of+pd-1%28high%29+cd4%28%2B%29cd8%28%2B%29+t+cells+in+hepatocellular+carcinoma+by+single-cell+cytometry+and+transcriptome+sequencing%5BTitle%5D)+AND+%22Adv+Sci%22%5BJournal%5D)">Trajectory and functional analysis of pd-1(high) cd4(+)cd8(+) t cells in hepatocellular carcinoma by single-cell cytometry and transcriptome sequencing.</a> Adv Sci. 7 (13), 2000224(2020).</li>
<li>Song, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Protocol+for+isolating+single+cells+from+human+pancreatic+cancer+tissues+and+analyzing+major+immune+cell+populations+using+flow+cytometry%5BTitle%5D)+AND+%22STAR+Protoc%22%5BJournal%5D)">Protocol for isolating single cells from human pancreatic cancer tissues and analyzing major immune cell populations using flow cytometry.</a> STAR Protoc. 4 (4), 102679(2023).</li>
<li>Simoni, Y., Chng, M. H. Y., Li, S., Fehlings, M., Newell, E. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Mass+cytometry%3A+A+powerful+tool+for+dissecting+the+immune+landscape%5BTitle%5D)+AND+%22Curr+Opin+Immunol%22%5BJournal%5D)">Mass cytometry: A powerful tool for dissecting the immune landscape.</a> Curr Opin Immunol. 51, 187-196 (2018).</li>
<li>Comi, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Coexpression+of+cd163+and+cd141+identifies+human+circulating+il-10-producing+dendritic+cells+%28dc-10%29%5BTitle%5D)+AND+%22Cell+Mol+Immunol%22%5BJournal%5D)">Coexpression of cd163 and cd141 identifies human circulating il-10-producing dendritic cells (dc-10).</a> Cell Mol Immunol. 17 (1), 95-107 (2020).</li>
<li>Fan, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((High-dimensional+single-cell+analysis+delineates+peripheral+immune+signature+of+coronary+atherosclerosis+in+human+blood%5BTitle%5D)+AND+%22Theranostics%22%5BJournal%5D)">High-dimensional single-cell analysis delineates peripheral immune signature of coronary atherosclerosis in human blood.</a> Theranostics. 12 (15), 6809-6825 (2022).</li>
<li>Sheng, J., et al. Human endogenous retrovirus activation contributes to biliary atresia pathogenesis through re-education of resident macrophages. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aSheng%2C+J+%22Biorxiv%22">Biorxiv</a>. , (2022).</li>
<li>Terekhova, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Single-cell+atlas+of+healthy+human+blood+unveils+age-related+loss+of+nkg2c%28%2B%29gzmb%28-%29cd8%28%2B%29+memory+t+cells+and+accumulation+of+type+2+memory+t+cells%5BTitle%5D)+AND+%22Immunity%22%5BJournal%5D)">Single-cell atlas of healthy human blood unveils age-related loss of nkg2c(+)gzmb(-)cd8(+) memory t cells and accumulation of type 2 memory t cells.</a> Immunity. 56 (12), 2836-2854.e9 (2023).</li>
<li>Sathaliyawala, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Distribution+and+compartmentalization+of+human+circulating+and+tissue-resident+memory+t+cell+subsets%5BTitle%5D)+AND+%22Immunity%22%5BJournal%5D)">Distribution and compartmentalization of human circulating and tissue-resident memory t cell subsets.</a> Immunity. 38 (1), 187-197 (2013).</li>
<li>Lee, S., Vu, H. M., Lee, J. H., Lim, H., Kim, M. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Advances+in+mass+spectrometry-based+single+cell+analysis%5BTitle%5D)+AND+%22Biology%22%5BJournal%5D)">Advances in mass spectrometry-based single cell analysis.</a> Biology. 12 (3), 395(2023).</li>
<li>Iyer, A., Hamers, A. A. J., Pillai, A. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Cytof+for+the+masses%5BTitle%5D)+AND+%22Front+Immunol%22%5BJournal%5D)">Cytof for the masses.</a> Front Immunol. 13, 815828(2022).</li>
<li>Yuan, X., Wang, J., Huang, Y., Shangguan, D., Zhang, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Single-cell+profiling+to+explore+immunological+heterogeneity+of+tumor+microenvironment+in+breast+cancer%5BTitle%5D)+AND+%22Front+Immunol%22%5BJournal%5D)">Single-cell profiling to explore immunological heterogeneity of tumor microenvironment in breast cancer.</a> Front Immunol. 12, 643692(2021).</li>
<li>Serban, G. M., Mănescu, I. B., Manu, D. R., Dobreanu, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Optimization+of+a+density+gradient+centrifugation+protocol+for+isolation+of+peripheral+blood+mononuclear+cells%5BTitle%5D)+AND+%22Acta+Marisiensis+-+Seria+Medica%22%5BJournal%5D)">Optimization of a density gradient centrifugation protocol for isolation of peripheral blood mononuclear cells.</a> Acta Marisiensis - Seria Medica. 64 (2), 83-90 (2018).</li>
<li>Shcherbakova, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Factors+affecting+cell+viability+during+the+enzymatic+dissociation+of+human+endocrine+tumor+tissues%5BTitle%5D)+AND+%22Biology+%28Basel%29%22%5BJournal%5D)">Factors affecting cell viability during the enzymatic dissociation of human endocrine tumor tissues.</a> Biology (Basel). 13 (9), 665(2024).</li>
<li>Devine, R. D., Alkhalaileh, H. S., Lyberger, J. M., Behbehani, G. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Alternative+methods+of+viability+determination+in+single+cell+mass+cytometry%5BTitle%5D)+AND+%22Cytometry+A%22%5BJournal%5D)">Alternative methods of viability determination in single cell mass cytometry.</a> Cytometry A. 99 (10), 1042-1053 (2021).</li>
<li>Gonzalez, V. D., Huang, Y. W., Fantl, W. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Mass+cytometry+for+the+characterization+of+individual+cell+types+in+ovarian+solid+tumors%5BTitle%5D)+AND+%22Methods+Mol+Biol%22%5BJournal%5D)">Mass cytometry for the characterization of individual cell types in ovarian solid tumors.</a> Methods Mol Biol. 2424, 59-94 (2022).</li>
<li>Zabransky, D. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Profiling+of+syngeneic+mouse+hcc+tumor+models+as+a+framework+to+understand+anti-pd-1+sensitive+tumor+microenvironments%5BTitle%5D)+AND+%22Hepatology%22%5BJournal%5D)">Profiling of syngeneic mouse hcc tumor models as a framework to understand anti-pd-1 sensitive tumor microenvironments.</a> Hepatology. 77 (5), 1566-1579 (2023).</li>
<li>Levine, L. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Single-cell+analysis+by+mass+cytometry+reveals+metabolic+states+of+early-activated+cd8%28%2B%29+t+cells+during+the+primary+immune+response%5BTitle%5D)+AND+%22Immunity%22%5BJournal%5D)">Single-cell analysis by mass cytometry reveals metabolic states of early-activated cd8(+) t cells during the primary immune response.</a> Immunity. 54 (4), 829-844.e5 (2021).</li>
<li>Lv, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Immunotherapy%3A+Reshape+the+tumor+immune+microenvironment%5BTitle%5D)+AND+%22Front+Immunol%22%5BJournal%5D)">Immunotherapy: Reshape the tumor immune microenvironment.</a> Front Immunol. 13, 844142(2022).</li>
<li>Zhou, Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Deciphering+the+tumor+immune+microenvironment+from+a+multidimensional+omics+perspective%3A+Insight+into+next-generation+car-t+cell+immunotherapy+and+beyond%5BTitle%5D)+AND+%22Mol+Cancer%22%5BJournal%5D)">Deciphering the tumor immune microenvironment from a multidimensional omics perspective: Insight into next-generation car-t cell immunotherapy and beyond.</a> Mol Cancer. 23 (1), 131(2024).</li>
<li>Hsieh, W. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Spatial+multi-omics+analyses+of+the+tumor+immune+microenvironment%5BTitle%5D)+AND+%22J+Biomed+Sci%22%5BJournal%5D)">Spatial multi-omics analyses of the tumor immune microenvironment.</a> J Biomed Sci. 29 (1), 96(2022).</li>
<li>Wang, Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Gdf15+induces+immunosuppression+via+cd48+on+regulatory+t+cells+in+hepatocellular+carcinoma%5BTitle%5D)+AND+%22J+Immunother+Cancer%22%5BJournal%5D)">Gdf15 induces immunosuppression via cd48 on regulatory t cells in hepatocellular carcinoma.</a> J Immunother Cancer. 9 (9), e002787(2021).</li>
<li>Krams, S. M., Schaffert, S., Lau, A. H., Martinez, O. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Applying+mass+cytometry+to+the+analysis+of+lymphoid+populations+in+transplantation%5BTitle%5D)+AND+%22Am+J+Transplant%22%5BJournal%5D)">Applying mass cytometry to the analysis of lymphoid populations in transplantation.</a> Am J Transplant. 17 (8), 1992-1999 (2017).</li>
</ol>

<table><tbody><tr><td>1&times;PBS</td><td>HyClone</td><td>SH30256.01</td><td></td></tr><tr><td>10&times;PBS</td><td>HyClone</td><td>SH30256.01</td><td></td></tr><tr><td>100 mm&times;20 mm tissue-culture-treated culture dish</td><td>Corning</td><td>430167</td><td></td></tr><tr><td>1000 mL pipette tips</td><td>Rainin</td><td>30389218</td><td></td></tr><tr><td>15 mL centrifuge tube</td><td>NEST</td><td>601052</td><td></td></tr><tr><td>200 mL pipette tips</td><td>Rainin</td><td>30389241</td><td></td></tr><tr><td>40 mm nylon cell strainer/70-mm nylon cell strainer</td><td>Falcon</td><td>352340</td><td></td></tr><tr><td>50 mL centrifuge tube</td><td>NEST</td><td>602052</td><td></td></tr><tr><td>70 &mu;m syringe fifilter</td><td>Sangon Biotech</td><td>F613462-9001</td><td></td></tr><tr><td>Anti-Human CCR2 Antibody (clone: K036C2)</td><td>BioLegend</td><td>357224</td><td></td></tr><tr><td>Anti-Human CCR3 Antibody (clone: 5E8)</td><td>BioLegend</td><td>310724</td><td></td></tr><tr><td>Anti-Human CCR7 Antibody (clone: G043H7)</td><td>BioLegend</td><td>353240</td><td></td></tr><tr><td>Anti-Human CD103 Antibody (clone: Ber-ACT8)</td><td>BioLegend</td><td>350202</td><td></td></tr><tr><td>Anti-Human CD115 Antibody (clone: 9-4D2-1E4)</td><td>BioLegend</td><td>347314</td><td></td></tr><tr><td>Anti-Human CD117 Antibody (clone: 104D2)</td><td>BioLegend</td><td>313201</td><td></td></tr><tr><td>Anti-human CD11b Antibody (clone: 1CRF44)&nbsp;</td><td>BD</td><td>562721</td><td></td></tr><tr><td>Anti-human CD11c Antibody (clone: B-ly6)&nbsp;</td><td>BD</td><td>563026</td><td></td></tr><tr><td>Anti-Human CD123 Antibody (clone: 6H6)</td><td>BioLegend</td><td>306002</td><td></td></tr><tr><td>Anti-Human CD127 Antibody (clone: A019D5)</td><td>BioLegend</td><td>351337</td><td></td></tr><tr><td>Anti-Human CD13 Antibody (clone: WM19)</td><td>BioLegend</td><td>301701</td><td></td></tr><tr><td>Anti-Human CD138 Antibody (clone: MI15)</td><td>BioLegend</td><td>356535</td><td></td></tr><tr><td>Anti-human CD14 Antibody (clone: HCD14)</td><td>BioLegend</td><td>325604</td><td></td></tr><tr><td>Anti-Human CD141 Antibody (clone: M80)</td><td>BioLegend</td><td>344102</td><td></td></tr><tr><td>Anti-Human CD15 Antibody (clone: QA19A61)</td><td>BioLegend</td><td>376302</td><td></td></tr><tr><td>Anti-human CD16 Antibody (clone: B7311)&nbsp;</td><td>BD</td><td>561313</td><td></td></tr><tr><td>Anti-Human CD161 Antibody (clone: HP-3G10)</td><td>BioLegend</td><td>339902</td><td></td></tr><tr><td>Anti-Human CD163 Antibody (clone: GHI/61)</td><td>BioLegend</td><td>333603</td><td></td></tr><tr><td>Anti-Human CD169 Antibody (clone: 7-239)</td><td>BioLegend</td><td>346002</td><td></td></tr><tr><td>Anti-human CD19 Antibody (clone: HIB19)</td><td>BioLegend</td><td>302226</td><td></td></tr><tr><td>Anti-Human CD1c Antibody (clone: L161)</td><td>BioLegend</td><td>331501</td><td></td></tr><tr><td>Anti-Human CD20 Antibody (clone: 2H7)</td><td>BioLegend</td><td>302301</td><td></td></tr><tr><td>Anti-Human CD206 Antibody (clone: 15-2)</td><td>BioLegend</td><td>321151</td><td></td></tr><tr><td>Anti-Human CD24 Antibody (clone: ML5)</td><td>BioLegend</td><td>311129</td><td></td></tr><tr><td>Anti-Human CD25 Antibody (clone: BC96)</td><td>BioLegend</td><td>302624</td><td></td></tr><tr><td>Anti-human CD3 Antibody (clone: UCHT1)&nbsp;</td><td>BD</td><td>555916</td><td></td></tr><tr><td>Anti-Human CD31 Antibody (clone: W18200D)</td><td>BioLegend</td><td>375902</td><td></td></tr><tr><td>Anti-Human CD32 Antibody (clone: FUN-2)</td><td>BioLegend</td><td>303232</td><td></td></tr><tr><td>Anti-Human CD326 Antibody (clone: CO17-1A)</td><td>BioLegend</td><td>369812</td><td></td></tr><tr><td>Anti-Human CD33 Antibody (clone: WM53)</td><td>BioLegend</td><td>303402</td><td></td></tr><tr><td>Anti-human CD4 Antibody (clone: L200)&nbsp;</td><td>BD</td><td>563094</td><td></td></tr><tr><td>Anti-Human CD45 Antibody (clone: HI30)&nbsp;</td><td>BD</td><td>563716</td><td></td></tr><tr><td>Anti-Human CD45RO Antibody (clone: UCHL1)</td><td>BioLegend</td><td>304220</td><td></td></tr><tr><td>Anti-human CD56 Antibody (clone: 5.1H11)</td><td>BioLegend</td><td>362510</td><td></td></tr><tr><td>Anti-Human CD64 Antibody (clone: S18012C)</td><td>BioLegend</td><td>399502</td><td></td></tr><tr><td>Anti-Human CD66b Antibody (clone: 6/40c)</td><td>BioLegend</td><td>392917</td><td></td></tr><tr><td>Anti-human CD68 Antibody (clone: Y1/82A)</td><td>BioLegend</td><td>333808</td><td></td></tr><tr><td>Anti-Human CD69 Antibody (clone: FN50)</td><td>BioLegend</td><td>310902</td><td></td></tr><tr><td>Anti-Human CD7 Antibody (clone: 4H9/CD7)</td><td>BioLegend</td><td>395602</td><td></td></tr><tr><td>Anti-human CD8 Antibody (clone: RPA-T8)&nbsp;</td><td>BD</td><td>557750</td><td></td></tr><tr><td>Anti-Human CD80 Antibody (clone: W17149D)</td><td>BioLegend</td><td>375402</td><td></td></tr><tr><td>Anti-Human CD86 Antibody (clone: BU63)</td><td>BioLegend</td><td>374202</td><td></td></tr><tr><td>Anti-Human FOXP3 Antibody (clone: 206D)</td><td>BioLegend</td><td>320101</td><td></td></tr><tr><td>Anti-Human HLA_ABC Antibody (clone: W6/32)</td><td>BioLegend</td><td>311426</td><td></td></tr><tr><td>Anti-human HLA-DR Antibody (clone: L243)</td><td>BioLegend</td><td>307650</td><td></td></tr><tr><td>Anti-Human IgD Antibody (clone: IA6-2)</td><td>BioLegend</td><td>348211</td><td></td></tr><tr><td>Anti-Human Ki67 Antibody (clone: Ki-67)</td><td>BioLegend</td><td>350501</td><td></td></tr><tr><td>Anti-Human PD_L2 Antibody (clone: MH22B2)</td><td>BD</td><td>567783</td><td></td></tr><tr><td>Anti-Human PD1 Antibody (clone: EH12.2H7)</td><td>BioLegend</td><td>329951</td><td></td></tr><tr><td>Anti-Human PDL1 Antibody (clone: MIH2)</td><td>BioLegend</td><td>393602</td><td></td></tr><tr><td>Anti-human TCR-&gamma;&delta; Antibody (clone: B1)&nbsp;</td><td>BD</td><td>74

análisis por citometría de masas de las respuestas inmunitarias sistémicas y locales en el carcinoma hepatocelular

Mass Cytometry Analysis of Systemic and Local Immune Responses in Hepatocellular Carcinoma

Análisis por citometría de masas de las respuestas inmunitarias sistémicas y locales en el carcinoma hepatocelular

Hepatocellular carcinoma (HCC) is one of the most common and deadliest forms of liver cancer worldwide. Despite advances in treatment, the prognosis for ...

mass-cytometry-analysis-systemic-local-immune-responses

Research

Investigación

JoVE Journal

Biology

Biología

Aquí se describe un método para separar y enriquecer los componentes del microambiente tumoral inmune y no inmune en tumores subcutáneos establecidos. Esta técnica permite el análisis separado de infiltrado inmune tumor y fracciones de tumor no inmune que pueden permitir una caracterización completa del microambiente tumoral inmune.

Enriquecimiento y caracterización de los microambientes Tumor inmunes y no inmunes en tumores murinos subcutáneos establecidos

Aquí describimos un enfoque proteómico unicelular para evaluar inmunológico fenotípico y funcional (inducción de citocinas intracelulares) alteraciones en las muestras de sangre periférica, analizadas mediante citometría de masas.

Análisis unicelular de Immunophenotype y producción de citoquinas en sangre periférica por citometría de masas

Aquí, describimos el desarrollo de un modelo murino clínicamente relevante de cáncer de hígado recapitulando las características inmunitarias típicas del cáncer hepatocelular (HCC).

Un modelo de ratón ortotópico inducido por hepatocitos oncogénicos que surge en el ajuste de la inflamación hepática y la fibrosis

La evasión de la erradicación mediada por células mortales por asesinos naturales (NK) por las células cancerosas es importante para la iniciación y progresión del cáncer. Aquí, presentamos dos protocolos no basados en la radiactividad para evaluar la citotoxicidad mediada por células NK hacia las células tumorales hepáticas. Además, se presenta un tercer protocolo para analizar la migración de células NK.

Medición de la citotoxicidad y migración mediadas por células asesinas naturales en el contexto de las células tumorales hepáticas

Aquí, describimos una estrategia simple y accesible para visualizar, cuantificar y mapear células inmunitarias en secciones de tejido tumoral incrustado en parafina fija de formalina. Esta metodología combina las técnicas existentes de análisis de imágenes y digitales con el propósito de ampliar la capacidad de multiplexación y el análisis multiparámetro de ensayos de imágenes.

Visualización, cuantificación y mapeo de poblaciones de células inmunes en el microambiente tumoral

Faltan modelos integrales in vitro que recapitulen fielmente la enfermedad humana relevante. El presente estudio presenta la creación y cultivo de esferoides tumorales tridimensionales (3D), una herramienta in vitro fiable para estudiar la interacción tumor-estroma en el carcinoma hepatocelular humano.

Un modelo esferoide tridimensional para investigar la interacción tumor-estroma en el carcinoma hepatocelular

Este protocolo describe la generación de ratones del sistema inmune humano (HIS) para estudios de inmunooncología. Las instrucciones y consideraciones en el uso de este modelo para probar inmunoterapias humanas en tumores humanos implantados en este modelo se presentan con énfasis en la caracterización de la respuesta del sistema inmune humano al tumor.

Prueba de inmunoterapia contra el cáncer en un modelo de ratón humanizado con tumores humanos

Aquí, detallamos un protocolo simple, rápido y confiable de citometría de flujo multiparamétrica y asistida por tumores en un chip para monitorear y caracterizar los pasos que constituyen el ciclo de inmunidad al cáncer. De hecho, una comprensión profunda de las interacciones entre el cáncer y las células inmunitarias proporciona información crítica para burlar a los tumores y guiar la atención clínica.

Cocultivo de sistemas in vitro para reproducir el ciclo cáncer-inmunidad

La presencia de mastocitos en el margen interno y en las áreas peritumorales del carcinoma hepatocelular después de la resección confiere un pronóstico favorable. Este estudio avala el software de análisis de imágenes QuPath como una plataforma prometedora que podría satisfacer la necesidad de reproducibilidad, consistencia y precisión en la patología digital.

Los mastocitos en el microambiente del carcinoma hepatocelular confieren un pronóstico favorable: un estudio retrospectivo con el software de análisis de imágenes QuPath 

Este video muestra la disección y remoción del timo fetal y la preparación de cultivos ex vivo de 2-dGuo tratados con el timo.

Preparación de 2-dGuo cultivos tratados con órganos timo

Aprende a analizar respuestas inmunitarias sistémicas y locales en carcinoma hepatocelular mediante citometría de masas. Este protocolo identifica 14 tipos celulares distintos en muestras de PBMC para estudiar mecanismos de evasión tumoral.

Análisis por citometría de masas de las respuestas inmunitarias sistémicas y locales en el carcinoma hepatocelular

Explorar más videos