Enfoques de biología estructural y química analítica para caracterizar las enzimas metabólicas de glucósidos C en la microbiota intestinal humana

0:19

Construction of Expression Vector

1:23

Purification of Protein

2:56

Circular Dichroism (CD) Spectrum Data Collection

3:37

Protein Crystal Growth and Optimization

4:27

Data Collection and Structure Determination of Protein Crystals

5:14

Monitoring of DgpA/B/C Activity by HPLC

6:39

Monitoring of DgpA Activity with 2,6-dichlorophenol indophenol (DCPIP) Assay

7:15

Determination of Inhibition Rate of Glucose on the C-glycosidic Bond Cleavage Reaction of Puerarin

7:51

Determination of Kinetic Parameters

8:38

Preparation of Reaction Intermediate Product

9:13

Characterization of Reaction Intermediate Product with LC-MS

9:58

Characterization of Reaction Intermediate Product with NMR

10:40

Representative Results

12:52

Conclusion