Name: Interfaces eléctricas y optoelectrónicas bidireccionales en corazones de rata ex vivo sanos e isquémicos (Video) | JoVE Journal
Uploaded: 2025-07-18T13:20:00.000+00:00
Duration: 8 min 33 s
Description: Aprende a evaluar materiales bioelectrónicos en corazones de rata ex vivo mediante interfaces ópticas y eléctricas bidireccionales. Este protocolo permite estudiar estimulación cardíaca, registro de señales y modelado de infarto miocárdico.

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5 minutes

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</ol>

<table><tbody><tr><td>Cabezal de 16 canales</td><td>Tecnologías Intan</td><td>C3334</td><td></td></tr><tr><td>Cloruro de 2,3,5-trifeniltetrazolio</td><td>Sigma Aldrich</td><td>T8877-5G</td><td>cloruro de trifeniltetrazolio </td></tr><tr><td>2-Propanol (IPA) </td><td>Fischer Scientific</td><td>A451-4</td><td></td></tr><tr><td>Láser de 635 nm, 500 mW</td><td>Resplandor láser</td><td>NA</td><td></td></tr><tr><td>Acetona</td><td>Fischer Scientific</td><td>A18-4</td><td></td></tr><tr><td>Evaporador de haz de electrones Angstrom Evovac</td><td>Angstrom</td><td>NA</td><td></td></tr><tr><td>AZ 300MIF Revelador</td><td>Microquímicos</td><td>18441123163</td><td>Desarrollador</td></tr><tr><td>AZ nlof 2020 </td><td>Microquímicos</td><td>1A002020</td><td></td></tr><tr><td>AZ NMP </td><td>Microquímicos</td><td>NA</td><td>Removedor de fotorresistencia</td></tr><tr><td>AZ40XT-11D </td><td>Microquímicos</td><td>1000300</td><td>Fotorresistencia</td></tr><tr><td>HF amortiguado</td><td>Thermo Scientific</td><td>AA44627K2</td><td></td></tr><tr><td>CaCl2.2H2O</td><td>Sigma Aldrich</td><td>(C3881)</td><td></td></tr><tr><td>Láser de CO2 </td><td>Sistemas láser universales</td><td>VLS 4.60</td><td></td></tr><tr><td>Pintura conductora de plata</td><td>PELCO</td><td>16062</td><td>Adhesión conductiva entre el electrodo y el cable de Cu de conexión</td></tr><tr><td>Alambre de cobre encapsulado 0,1 mm</td><td>Amazona</td><td>https://www.amazon.com/Enameled-Temperature-Resistance-Transformers-Inductors/dp/ B09TNM51S5/ref=asc_df_B09 TNM51S5?mcid=155de761ea4 c389ea86d45ba61a83e8d& etiqueta =hyprod-20& linkCode=df0& hvadid =693675076770& hvpos=& hvnetw =g& hvrand=664358781094905 4431& hvpone=& hvptwo=& hvqmt =& hvdev=c& hvdvcmdl=& hvlocint =& hvlocphy=9021716& hvtargid= pla-1878323158943& psc=1</td><td></td></tr><tr><td>Digitalizador Digidata 1550</td><td>Dispositivos moleculares</td><td>NA</td><td></td></tr><tr><td>Glucosa</td><td>Sigma Aldrich</td><td>G7021</td><td></td></tr><tr><td>HBSS, sin calcio, sin magnesio, sin rojo de fenol</td><td>Thermo Scientific</td><td>14175095</td><td></td></tr><tr><td>Escritor directo Heidelberg MLA150</td><td>Heidelberg</td><td>NA</td><td></td></tr><tr><td>heparina (1000 UI/kg)</td><td>Productos farmacéuticos de sagent</td><td>1009655</td><td></td></tr><tr><td>HEPES</td><td>Sigma Aldrich</td><td>(H4034)</td><td></td></tr><tr><td>Hexano </td><td>Sigma Aldrich</td><td>52750-10ML</td><td>Solvente</td></tr><tr><td>HF</td><td>Fisher Scientific</td><td>A513-500</td><td>Grado de tracemetal</td></tr><tr><td>Horno HMDS Vapor Prime</td><td>Sistemas de ingeniería de rendimiento</td><td>NA</td><td></td></tr><tr><td>HNO3</td><td>Fisher Scientific</td><td>A509P500</td><td>Grado de tracemetal</td></tr><tr><td>Laminadora en caliente</td><td>Depósito de oficinas</td><td>L410-A</td><td>Encapsulación multicapa </td></tr><tr><td>Sistemas Intan RHD</td><td>Tecnologías Intan</td><td>C3100</td><td></td></tr><tr><td>ECG iWorx</td><td>iWorx</td><td> IA-400D</td><td></td></tr><tr><td>Sonda iWorx LVD</td><td>iWorx</td><td>BP-100</td><td></td></tr><tr><td>Preamplificador iWorx</td><td>iWorx</td><td>C-ISO-256</td><td></td></tr><tr><td>Kcl</td><td>Sigma Aldrich</td><td>(P4504)</td><td></td></tr><tr><td>Globo de látex (talla 4)</td><td>Radnoti</td><td>170404</td><td></td></tr><tr><td>MgCl2.7H2O</td><td>Sigma Aldrich</td><td>(M9272)</td><td></td></tr><tr><td>NaCl</td><td>Sigma Aldrich</td><td>(S9625)</td><td></td></tr><tr><td>Solución que contiene Nafion 117</td><td>Sigma Aldrich</td><td>31175-20-9</td><td>Reactivo de fijación para patrón de carbono nanoporoso</td></tr><tr><td>NaH2PO4</td><td>Sigma Aldrich</td><td>(S0751)</td><td></td></tr><tr><td>NaOH</td><td>Sigma Aldrich</td><td>221465</td><td></td></tr><tr><td>PDMS</td><td>Fisher Scientific</td><td>NC9285739</td><td></td></tr><tr><td>Grabador de fluoruro de plasma acoplado inductivamente Plasma-Therm</td><td>Plasma-Therm</td><td>NA</td><td></td></tr><tr><td>PMMA (495 A6) </td><td>Microquímicos</td><td>M130006 </td><td></td></tr><tr><td>poli (dianhídrido piromelítico-co-4,4'-oxidianilina)</td><td>Sigma Aldrich</td><td>575801</td><td>Precursor de PI</td></tr><tr><td>Poli (etileno-co-acetato de vinilo)</td><td>Sigma Aldrich</td><td>437247-250G</td><td>Polímero para infiltración</td></tr><tr><td>Poli (alcohol vinílico)</td><td>Sigma Aldrich</td><td>341584-25G</td><td></td></tr><tr><td>Tereftalato de polietileno (PET)</td><td>Amazona</td><td>KS-6304-21-11</td><td>Hoja de PET transparente tipo D .0005" de espesor x 27" de ancho x 10 pies de longitud 1 pc</td></tr><tr><td>Potenciostato</td><td>Biológico</td><td>SP-200</td><td>Estimulación eléctrica</td></tr><tr><td>resistir el horno</td><td> Envío</td><td>LCC1-15-5</td><td></td></tr><tr><td>Microscopía electrónica de barrido</td><td>Carl Zeiss</td><td>Esmerejón</td><td>Instrumento para la caracterización de la morfología</td></tr><tr><td>oblea de silicio </td><td>Materiales electrónicos NOVA</td><td>HS39626</td><td></td></tr><tr><td>Adhesivo de silicona</td><td>WPI</td><td>KWIK-SIL-S</td><td>Encapsulación</td></tr><tr><td>Borato de sodio</td><td>Sigma Aldrich</td><td>SX0355</td><td></td></tr><tr><td>Oblea SOI</td><td>Ultrasil</td><td>3-10541</td><td>Tipo-Oriente: P / B (100) DT: 7 +/- 1um DR: <. 005 Ohmcm HT: 300 +/- 10um HR: 1-30 Ohmcm Caja: 1um +/- 5% UD-13998 / UH-13962</td></tr><tr><td>SU-8 2002</td><td>Microquímicos</td><td>Serie SU-8 2000</td><td></td></tr><tr><td>Desarrollador SU-8</td><td>Microquímicos</td><td>Serie SU-8 2000</td><td></td></tr><tr><td>Adhesivo de tejido húmedo</td><td>3M</td><td>1469SB</td><td></td></tr><tr><td>Papel de filtro cualitativo Whatman, Grado 1</td><td>Sigma Aldrich</td><td>WHA1001150</td><td>Sustrato para patrones de carbono</td></tr></tbody></table>

interfaces eléctricas y optoelectrónicas bidireccionales en corazones de rata ex vivo sanos e isquémicos

Bidirectional Electrical and Optoelectronic Interfaces in Healthy and Ischemic Ex Vivo Rat Hearts

Interfaces eléctricas y optoelectrónicas bidireccionales en corazones de rata ex vivo sanos e isquémicos

Electrophysiological studies are pivotal in exploring interactions between materials and cardiac biointerfaces. Recent advancements have introduced ...

bidirectional-electrical-optoelectronic-interfaces-healthy-ischemic

Research

Investigación

JoVE Journal

Bioengineering

Bioingeniería

En este artículo se describe una técnica de alta resolución temporal y espacial de la imagen ópticamente movimiento de acción potencial en la superficie de los corazones de rata Langendorff perfundidos con un tinte potenciométrica (di-8-ANEPPS).

Mapeo óptico de corazones de ratas Langendorff perfundidos

En este artículo se describen los procedimientos básicos para la realización de experimentos ópticos de asignación en el corazón de conejo Langendorff perfundidos con el sistema de imágenes panorámicas, y el doble (voltaje y calcio) modalidad de imagen.

Multiparamétrico óptico Cartografía del corazón de conejo Langendorff perfundidos

El objetivo de este estudio fue establecer un método para investigar la dinámica cardíaca utilizando un modelo animal traslacional. El enfoque experimental descrito incorpora optocardiografía de doble emisión junto con un estudio electrofisiológico para evaluar la actividad eléctrica en un modelo de corazón porcino aislado e intacto.

Estudios de Optocardiografía y Electrofisiología de Corazones Perfundidos Ex Vivo Langendorff

Este protocolo describe el procedimiento para secimiento y cultivo de rebanadas cardíacas humanas para pruebas de fármacos preclínicos y detalla el uso de mapeo óptico para registrar el voltaje transmembrano y las señales de calcio intracelular simultáneamente a partir de estas rebanadas.

Evaluación de electrofisiología cardíaca preclínica por doble voltaje y mapeo óptico de calcio de cortes cardíacos organotípicos humanos

Este artículo presenta un método eficiente para realizar isquemia miocárdica y posterior reperfusión crónica en ratas utilizando un enfoque mínimamente invasivo. Además, la función hemodinámica ventricular izquierda de las ratas se evalúa mediante ecocardiografía y métodos aislados del corazón de trabajo.

Método invasivo semiseminal para inducir el infarto de miocardio en ratas y la evaluación de la función cardíaca mediante un sistema cardíaco de trabajo aislado

Este trabajo informa un método para controlar el ritmo cardíaco de corazones murinos intactos de ratones transgénicos con canalrodopsina-2 (ChR2) utilizando fotoestimulación local con una matriz de micro-LED y mapeo óptico simultáneo del potencial de la membrana epicárdica.

Manejo avanzado del ritmo cardíaco mediante la aplicación de fotoestimulación optogenética multisitio en corazones murinos

Este protocolo introduce el mapeo óptico de doble colorante de corazones de ratón obtenidos de animales de tipo salvaje y knock-in afectados por taquicardia ventricular polimórfica catecolaminérgica, incluyendo mediciones electrofisiológicas de voltaje transmembrana y transitorios intracelulares de Ca2+ con alta resolución temporal y espacial.

Mapeo óptico de doble colorante de corazones a partir de ratones knock-in RyR2R2474S de taquicardia ventricular polimórfica catecolaminérgica

Este protocolo ilustra un método para la estimulación optogenética de neuronas cardíacas intrínsecas en corazones de ratones transgénicos. El enfoque descrito se utiliza para investigar la cinética de la activación súbita de las neuronas cardíacas en corazones perfundidos ex vivo y las interacciones entre la actividad colinérgica y catecolaminérgica.

Activación optogenética de neuronas autonómicas cardíacas intrínsecas en corazones de ratón perfundidos extirpados

Un sistema de cultivo en 3D para la hematopoyesis se describe utilizando sangre del cordón umbilical humano y células leucémicas de médula ósea. El método se basa en el uso de un poliuretano poroso sintético andamio revestido con proteínas de matriz extracelular. Este andamio es adaptable para acomodar una amplia gama de células.

Ex vivo mimetismo de la hematopoyesis humano normal y anormal

Prueba Microtracción ambientalmente controlado mecánicamente de adaptación nanocompuestos poliméricos para<em&gt; Ex vivo</em&gt; Caracterización

Aprende a evaluar materiales bioelectrónicos en corazones de rata ex vivo mediante interfaces ópticas y eléctricas bidireccionales. Este protocolo permite estudiar estimulación cardíaca, registro de señales y modelado de infarto miocárdico.

Interfaces eléctricas y optoelectrónicas bidireccionales en corazones de rata ex vivo sanos e isquémicos

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