Name: Ensayo de protección con amikacina para cuantificación y visualización de la invasión celular de Escherichia coli (Video) | JoVE Journal
Uploaded: 2026-07-03T12:40:00.000+00:00
Duration: 9 min 22 s
Description: Aprende el ensayo de protección con amikacina para cuantificar la invasión de células epiteliales por Escherichia coli adherente-invasiva. Este método alternativo supera las limitaciones del ensayo tradicional con gentamicina frente a cepas resistentes.

ai_agent_IP

ai_agent

maintenance

instantTrialDurationMinutes

isBiopharma

enableNewMyJoveSectionDesign

educationFeatures

seoMetaApi

ai_copilot_institution_blacklist

ai_chat_institution_blacklist

freeAccessWindow

ssoRoleCapture

scheduledMaintenanceBanner

joveLabsFeatures

disable_autoplay_for_bots

extended_trial_duration

5 minutes

<ol>
	<li>Bruner, L. P., White, A. M., Proksell, S. <a target="_blank" href="https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Inflammatory+bowel+disease.">Inflammatory bowel disease.</a> Prim Care. 50 (3), 411-427 (2023).</li><li>Richard, N., Savoye, G., Leboutte, M., Amamou, A., Ghosh, S., et al. <a target="_blank" href="https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Crohn's+disease:+Why+the+ileum?.">Crohn's disease: Why the ileum?.</a> World J Gastroenterol. 29 (21), 3222-3240 (2023).</li><li>Hracs, L., et al. <a target="_blank" href="https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Global+evolution+of+inflammatory+bowel+disease+across+epidemiologic+stages.">Global evolution of inflammatory bowel disease across epidemiologic stages.</a> Nature. 642 (8067), 458-466 (2025).</li><li>Lewis, J. D., Abreu, M. T. <a target="_blank" href="https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Diet+as+a+trigger+or+therapy+for+inflammatory+bowel+diseases.">Diet as a trigger or therapy for inflammatory bowel diseases.</a> Gastroenterology. 152 (2), 398-414.e6 (2017).</li><li>Khan, S., et al. <a target="_blank" href="https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dietary+simple+sugars+alter+microbial+ecology+in+the+gut+and+promote+colitis+in+mice.">Dietary simple sugars alter microbial ecology in the gut and promote colitis in mice.</a> Sci Transl Med. 12 (567), (2020).</li><li>Ma, X., et al. <a target="_blank" href="https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Gut+microbiota+in+the+early+stage+of+Crohn's+disease+has+unique+characteristics.">Gut microbiota in the early stage of Crohn's disease has unique characteristics.</a> Gut Pathog. 14 (1), 46 (2022).</li><li>Vester-Andersen, M. K., et al. <a target="_blank" href="https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Increased+abundance+of+proteobacteria+in+aggressive+Crohn's+disease+seven+years+after+diagnosis.">Increased abundance of proteobacteria in aggressive Crohn's disease seven years after diagnosis.</a> Sci Rep. 9 (1), 13473 (2019).</li><li>Boudeau, J., Glasser, A. L., Masseret, E., Joly, B., Darfeuille-Michaud, A. <a target="_blank" href="https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Invasive+ability+of+an+Escherichia+coli+strain+isolated+from+the+ileal+mucosa+of+a+patient+with+Crohn's+disease.">Invasive ability of an Escherichia coli strain isolated from the ileal mucosa of a patient with Crohn's disease.</a> Infect Immun. 67 (9), 4499-4509 (1999).</li><li>Darfeuille-Michaud, A., et al. <a target="_blank" href="https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=High+prevalence+of+adherent-invasive+Escherichia+coli+associated+with+ileal+mucosa+in+Crohn's+disease.">High prevalence of adherent-invasive Escherichia coli associated with ileal mucosa in Crohn's disease.</a> Gastroenterology. 127 (2), 412-421 (2004).</li><li>Barnich, N., et al. <a target="_blank" href="https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adherent-invasive+Escherichia+coli+in+Crohn's+disease:+the+25th+anniversary.">Adherent-invasive Escherichia coli in Crohn's disease: the 25th anniversary.</a> Gut. , (2025).</li><li>Kim, J., et al. <a target="_blank" href="https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Genetic+and+microbial+analysis+of+invasiveness+for+Escherichia+coli+strains+associated+with+inflammatory+bowel+disease.">Genetic and microbial analysis of invasiveness for Escherichia coli strains associated with inflammatory bowel disease.</a> Cell Mol Gastroenterol Hepatol. 19 (4), 101451 (2025).</li><li>Camprubi-Font, C., Martinez-Medina, M. <a target="_blank" href="https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Why+the+discovery+of+adherent-invasive+Escherichia+coli+molecular+markers+is+so+challenging?.">Why the discovery of adherent-invasive Escherichia coli molecular markers is so challenging?.</a> World J Biol Chem. 11 (1), 1-13 (2020).</li><li>Barnich, N., et al. <a target="_blank" href="https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=CEACAM6+acts+as+a+receptor+for+adherent-invasive+E.+coli,+supporting+ileal+mucosa+colonization+in+Crohn+disease.">CEACAM6 acts as a receptor for adherent-invasive E. coli, supporting ileal mucosa colonization in Crohn disease.</a> J Clin Invest. 117 (6), 1566-1574 (2007).</li><li>Boudeau, J., Barnich, N., Darfeuille-Michaud, A. <a target="_blank" href="https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Type+1+pili-mediated+adherence+of+Escherichia+coli+strain+LF82+isolated+from+Crohn's+disease+is+involved+in+bacterial+invasion+of+intestinal+epithelial+cells.">Type 1 pili-mediated adherence of Escherichia coli strain LF82 isolated from Crohn's disease is involved in bacterial invasion of intestinal epithelial cells.</a> Mol Microbiol. 39 (5), 1272-1284 (2001).</li><li>Hase, K., et al. <a target="_blank" href="https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Uptake+through+glycoprotein+2+of+FimH(+)+bacteria+by+M+cells+initiates+mucosal+immune+response.">Uptake through glycoprotein 2 of FimH(+) bacteria by M cells initiates mucosal immune response.</a> Nature. 462 (7270), 226-230 (2009).</li><li>Chassaing, B., et al. <a target="_blank" href="https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Crohn+disease--associated+adherent-invasive+E.+coli+bacteria+target+mouse+and+human+Peyer's+patches+via+long+polar+fimbriae.">Crohn disease--associated adherent-invasive E. coli bacteria target mouse and human Peyer's patches via long polar fimbriae.</a> J Clin Invest. 121 (3), 966-975 (2011).</li><li>Glasser, A. L., Boudeau, J., Barnich, N., Perruchot, M. H., Colombel, J. F., et al. <a target="_blank" href="https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adherent+invasive+Escherichia+coli+strains+from+patients+with+Crohn's+disease+survive+and+replicate+within+macrophages+without+inducing+host+cell+death.">Adherent invasive Escherichia coli strains from patients with Crohn's disease survive and replicate within macrophages without inducing host cell death.</a> Infect Immun. 69 (9), 5529-5537 (2001).</li><li>Bringer, M. A., Billard, E., Glasser, A. L., Colombel, J. F., Darfeuille-Michaud, A. <a target="_blank" href="https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Replication+of+Crohn's+disease-associated+AIEC+within+macrophages+is+dependent+on+TNF-alpha+secretion.">Replication of Crohn's disease-associated AIEC within macrophages is dependent on TNF-alpha secretion.</a> Lab Invest. 92 (3), 411-419 (2012).</li><li>Ristuccia, A. M., Cunha, B. A. <a target="_blank" href="https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+overview+of+amikacin.">An overview of amikacin.</a> Ther Drug Monit. 7 (1), 12-25 (1985).</li><li>Sambuy, Y., De Angelis, I., Ranaldi, G., Scarino, M. L., Stammati, A., et al. <a target="_blank" href="https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Caco-2+cell+line+as+a+model+of+the+intestinal+barrier:+influence+of+cell+and+culture-related+factors+on+Caco-2+cell+functional+characteristics.">The Caco-2 cell line as a model of the intestinal barrier: influence of cell and culture-related factors on Caco-2 cell functional characteristics.</a> Cell Biol Toxicol. 21 (1), 1-26 (2005).</li><li>Vllasaliu, D., Falcone, F. H., Stolnik, S., Garnett, M. <a target="_blank" href="https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Basement+membrane+influences+intestinal+epithelial+cell+growth+and+presents+a+barrier+to+the+movement+of+macromolecules.">Basement membrane influences intestinal epithelial cell growth and presents a barrier to the movement of macromolecules.</a> Exp Cell Res. 323 (1), 218-231 (2014).</li><li>Wine, E., Ossa, J. C., Gray-Owen, S. D., Sherman, P. M. <a target="_blank" href="https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adherent-invasive+Escherichia+coli,+strain+LF82+disrupts+apical+junctional+complexes+in+polarized+epithelia.">Adherent-invasive Escherichia coli, strain LF82 disrupts apical junctional complexes in polarized epithelia.</a> BMC Microbiol. 9, 180 (2009).</li><li>Jamur, M. C., Oliver, C. <a target="_blank" href="https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Permeabilization+of+cell+membranes.">Permeabilization of cell membranes.</a> Methods Mol Biol. 588, 63-66 (2010).</li><li>Delmas, J., et al. <a target="_blank" href="https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Metabolic+adaptation+of+adherent-invasive+Escherichia+coli+to+exposure+to+bile+salts.">Metabolic adaptation of adherent-invasive Escherichia coli to exposure to bile salts.</a> Sci Rep. 9 (1), 2175 (2019).</li><li>Zhang, S., et al. <a target="_blank" href="https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mucosal+metabolites+fuel+the+growth+and+virulence+of+E.+coli+linked+to+Crohn's+disease.">Mucosal metabolites fuel the growth and virulence of E. coli linked to Crohn's disease.</a> JCI Insight. 7 (10), (2022).</li><li>Viennois, E., et al. <a target="_blank" href="https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dietary+emulsifiers+directly+impact+adherent-invasive+E.+coli+gene+expression+to+drive+chronic+intestinal+inflammation.">Dietary emulsifiers directly impact adherent-invasive E. coli gene expression to drive chronic intestinal inflammation.</a> Cell Rep. 33 (1), 108229 (2020).</li><li>Chassaing, B., Etienne-Mesmin, L., Bonnet, R., Darfeuille-Michaud, A. <a target="_blank" href="https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bile+salts+induce+long+polar+fimbriae+expression+favouring+Crohn's+disease-associated+adherent-invasive+Escherichia+coli+interaction+with+Peyer's+patches.">Bile salts induce long polar fimbriae expression favouring Crohn's disease-associated adherent-invasive Escherichia coli interaction with Peyer's patches.</a> Environ Microbiol. 15 (2), 355-371 (2013).</li><li>Gotoh, K., et al. <a target="_blank" href="https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bile+acid-induced+virulence+gene+expression+of+Vibrio+parahaemolyticus+reveals+a+novel+therapeutic+potential+for+bile+acid+sequestrants.">Bile acid-induced virulence gene expression of Vibrio parahaemolyticus reveals a novel therapeutic potential for bile acid sequestrants.</a> PLoS One. 5 (10), e13365 (2010).</li><li>Bajunaid, W., et al. <a target="_blank" href="https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+T3SS+of+Shigella:+Expression,+structure,+function,+and+role+in+vacuole+escape.">The T3SS of Shigella: Expression, structure, function, and role in vacuole escape.</a> Microorganisms. 8 (12), (2020).</li><li>Poore, K., Lenneman, B. R., Faherty, C. S. <a target="_blank" href="https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Epithelial+cell+infection+analyses+with+Shigella.">Epithelial cell infection analyses with Shigella.</a> J Vis Exp. (204), e66426 (2024).</li><li>Chimalapati, S., Lafrance, A. E., Chen, L., Orth, K. <a target="_blank" href="https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Vibrio+parahaemolyticus:+Basic+techniques+for+growth,+genetic+manipulation,+and+analysis+of+virulence+factors.">Vibrio parahaemolyticus: Basic techniques for growth, genetic manipulation, and analysis of virulence factors.</a> Curr Protoc Microbiol. 59 (1), e131 (2020).</li><li>Rodrigues, E., Marzook, N. B., Pallett, M. A., Sateriale, A. <a target="_blank" href="https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Convergent+mechanisms+of+epithelial+cell+structure+manipulation+by+intestinal+pathogens.">Convergent mechanisms of epithelial cell structure manipulation by intestinal pathogens.</a> PLoS Pathog. 21 (7), e1013367 (2025).</li><li>Jaishankar, J., et al. <a target="_blank" href="https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+critical+role+for+Vibrio+parahaemolyticus+LPS+to+mediate+evasion+of+host+immune+response+during+infection.">A critical role for Vibrio parahaemolyticus LPS to mediate evasion of host immune response during infection.</a> Proc Natl Acad Sci U S A. 122 (33), e2426547122 (2025).</li><li>Cieza, R. J., Hu, J., Ross, B. N., Sbrana, E., Torres, A. G. <a target="_blank" href="https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+IbeA+invasin+of+adherent-invasive+Escherichia+coli+mediates+interaction+with+intestinal+epithelia+and+macrophages.">The IbeA invasin of adherent-invasive Escherichia coli mediates interaction with intestinal epithelia and macrophages.</a> Infect Immun. 83 (5), 1904-1918 (2015).</li><li>Liu, H., Semino-Mora, C., Dubois, A. <a target="_blank" href="https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mechanism+of+H.+pylori+intracellular+entry:+an+in+vitro+study.">Mechanism of H. pylori intracellular entry: an in vitro study.</a> Front Cell Infect Microbiol. 2, 13 (2012).</li><li>Heesemann, J., Laufs, R. <a target="_blank" href="https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Double+immunofluorescence+microscopic+technique+for+accurate+differentiation+of+extracellularly+and+intracellularly+located+bacteria+in+cell+culture.">Double immunofluorescence microscopic technique for accurate differentiation of extracellularly and intracellularly located bacteria in cell culture.</a> J Clin Microbiol. 22 (2), 168-175 (1985).</li><li>Lev-Tzion, R., Ledder, O., Shteyer, E., Tan, M. L. N., Uhlig, H. H., et al. <a target="_blank" href="https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Oral+vancomycin+and+gentamicin+for+treatment+of+very+early+onset+inflammatory+bowel+disease.">Oral vancomycin and gentamicin for treatment of very early onset inflammatory bowel disease.</a> Digestion. 95 (4), 310-313 (2017).</li></ol>

<table><tbody><tr><td>75 cm2 Matraz de cultivo de tejido</td><td>Fisher Scientific&nbsp;</td><td>FB012937</td><td></td></tr><tr><td>Amicacina disulfato&nbsp;</td><td>Research Products International&nbsp;</td><td>A20540-25.0</td><td></td></tr><tr><td>Antibiótico-Antimicótico (100x)</td><td>Gibco</td><td>15240062</td><td></td></tr><tr><td>Anticuerpo anti-E.coli</td><td>Abcam</td><td>ab137967</td><td></td></tr><tr><td>Placas de micropocillo BioLite - 6 pozos</td><td>Fisher Scientific&nbsp;</td><td>12-556-004</td><td></td></tr><tr><td>Albúmina sérica bovina</td><td>Sigma-Aldrich</td><td>A7906</td><td></td></tr><tr><td>Colágeno I, cola de rata, 100 mg</td><td>Corning</td><td>354236</td><td></td></tr><tr><td>Diapositiva de cámara de conteo celular Countess</td><td>Invitrogen</td><td>C10312</td><td></td></tr><tr><td>DMEM, alta glucosa</td><td>Gibco</td><td>11965118</td><td></td></tr><tr><td>Suero fetal bovino</td><td>Corning</td><td>35-011-CV</td><td></td></tr><tr><td>Ácido acético glacial</td><td>Sigma-Aldrich</td><td>A6283</td><td></td></tr><tr><td>Glicina ultrapura</td><td>USBiological</td><td>G8165</td><td></td></tr><tr><td>Anticuerpo secundario anti-Conejo IgG (H + L) cruzado adsorto, Alexa Fluor 488</td><td>Invitrogen</td><td>A-11008</td><td></td></tr><tr><td>Hoechst 33342, trihidrocloruro trihidrato</td><td>Invitrogen</td><td>H3570</td><td></td></tr><tr><td>Liofilchem&nbsp;MTS amikacina [AK] 0.016-256 &mu;g/mL</td><td>Fisher Scientific&nbsp;</td><td>22-777-704</td><td>Tiras de prueba MIC de amikacina</td></tr><tr><td>Solución de paraformaldehído, 4% en PBS</td><td>Fisher Scientific&nbsp;</td><td>J19943.K2</td><td></td></tr><tr><td>PBS, 1x</td><td>Corning</td><td>21-040-CV</td><td></td></tr><tr><td>Portaobjetos de vidrio alemán estéril recubiertos de poli-L-lisina para cultivo celular</td><td>Neuvitro</td><td>NC1327375</td><td></td></tr><tr><td>Montante antidesvanecimiento oro ProLong&nbsp;</td><td>Invitrogen</td><td>P36934</td><td></td></tr><tr><td>Rodamina-Falidina</td><td>Invitrogen</td><td>R415</td><td>Rojo-naranja 540/565 nm</td></tr><tr><td>Placas de cultivo de tejido estériles tratadas en superficie</td><td>Fisher Scientific&nbsp;</td><td>FB012929</td><td>Placas de 24 pozos</td></tr><tr><td>Triton X-100 (Electroforesis)</td><td>Fisher Scientific&nbsp;</td><td>BP151-100</td><td></td></tr><tr><td>Azúcar de tripán</td><td>Invitrogen</td><td>T10282</td><td></td></tr><tr><td>Tripsina-EDTA (0,25%), rojo fenol</td><td>Gibco</td><td>25200056</td><td></td></tr><tr><td>Tween-20</td><td>Fisher Scientific&nbsp;</td><td>BP337-100</td><td></td></tr></tbody></table>

ensayo de protección con amikacina para cuantificación y visualización de la invasión celular de escherichia coli

Amikacin Protection Assay for Quantification and Visualization of Escherichia coli Cell Invasion

Ensayo de protección con amikacina para cuantificación y visualización de la invasión celular de Escherichia coli

Epithelial cell invasion is a key phenotype for many enteric pathogens, including Salmonella, Shigella, and Vibrio. An emerging invasive pathogen is ...

amikacin-protection-assay-for-quantification-visualization

Research

Investigación

JoVE Journal

Immunology and Infection

Inmunología e Infección

Este video muestra la forma de medir la invasión de las células a través de insertos de cultivo celular utilizando una metodología basada en fluorescencia.

Un In vitro Tumor FluoroBlok invasión de ensayo

Un protocolo general para el estudio de la invasión de las células huésped por un patógeno bacteriano, centrándose...

La invasión de las células humanas por un patógeno bacteriano

Este protocolo es un ensayo simple adhesión bacteriana consiste en contar el número de unidades formadoras de colonias bacterianas que se adhieren a las células cultivadas. El ensayo es sólida, independiente de la adhesina estudiados, y numerosas variantes se utilizan en la mayoría de los laboratorios que trabajan en la patogénesis bacteriana.

Ensayo in vitro de la adhesión bacteriana a las células epiteliales de mamíferos

Los modelos de la invasión de células tumorales en tres dimensiones de la matriz extracelular para reflejar mejor...

Modelado y de imagen de 3 dimensiones invasión celular colectiva

Fuente: Cayron, J. y Lesterlin, C. Análisis multiescala del crecimiento bacteriano bajo tratamientos de estrés. J. Vis. Exp. (2019)Este video demuestra el procedimiento para evaluar la filamentación de E. coli y la replicación del ADN bajo tratamiento con antibióticos inhibidores de la división celular utilizando placas para medir la viabilidad y citometría de flujo para analizar el tamaño de la célula y el contenido de ADN.

Evaluación de la filamentación inducida por antibióticos en E. coli mediante ensayo de placa y citometría de flujo

Este video muestra la microscopía cuantitativa de lapso de tiempo para evaluar la diseminación de Listeria monocytogenes dependiente de la rigidez de la matriz extracelular a través de células endoteliales. Un hidrogel más suave facilita una diseminación bacteriana más rápida a través de las células endoteliales en comparación con sus homólogos más rígidos, lo que pone de manifiesto la influencia de la rigidez de la matriz en la diseminación bacteriana.

Microscopía cuantitativa de lapso de tiempo para evaluar la diseminación bacteriana dependiente de la rigidez de la matriz extracelular

Se describe un método para el seguimiento de la ruptura del endomembrane provocada por las bacterias...

Sola célula Medidas de Ruptura Vacuolar causadas por patógenos intracelulares

Se presenta un protocolo detallado de un modelo de ratón para enterohemorrágica e. coli (EHEC) colonización utilizando bacterias etiquetado bioluminiscencia. La detección de estas bacterias bioluminiscentes por un no-invasivo en vivo sistema de animales vivos de imagen puede avanzar nuestra comprensión actual de la colonización de EHEC.

Detección de la colonización de Escherichia Coli enterohemorrágica en anfitrión murino no invasiva In Vivo el sistema de bioluminiscencia

Hemos desarrollado un ensayo de base poliacrilamida formato multi-bien para sondear el efecto de la rigidez de la matriz extracelular en la infección bacteriana de las células adherentes. Este análisis es compatible con citometría de flujo, inmunotinción y microscopía de fuerza de tracción, lo que permite mediciones cuantitativas de las interacciones biomecánicas entre las células y su matriz extracelular, las bacterias patógenas.

Un ensayo de base poliacrilamida formato multi-bien para estudiar el efecto de la rigidez de la matriz extracelular en la infección bacteriana de las células adherentes

El actual protocolo describe un método integrado de investigación migración de la célula de cáncer y la invasión en una única plataforma en tiempo real, proporcionando una opción fácilmente reproducible y eficiente para estudiar la morfología y movilidad de la célula.

Un flujo de trabajo Simple migración/invasión usando a un sensor automatizado de células vivas

Este manuscrito describe un método para utilizar la microscopía de fluorescencia unicelular para visualizar y cuantificar la competencia bacteriana en el cocultivo.

Cuantificación de la competencia interbacteriana utilizando imágenes de fluorescencia unicelular

Aprende el ensayo de protección con amikacina para cuantificar la invasión de células epiteliales por Escherichia coli adherente-invasiva. Este método alternativo supera las limitaciones del ensayo tradicional con gentamicina frente a cepas resistentes.

Ensayo de protección con amikacina para cuantificación y visualización de la invasión celular de Escherichia coli

Explorar más videos