JOVE JOURNAL

Bioengineering

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Journal (Bioengineering)

Resistant Starch Content Measurement in Rice Varieties Developed Through Genome Editing

Chunxue Yan^1,2, Huicong Meng^1,2, Yanxin Pei^1,2, Wei Sun², Jinshan Zhang^1,2

¹Bellagen Biotechnology Co. Ltd, ²School of Life Sciences,Shandong Normal University

Journal (Bioengineering)

Breeding by Design for Functional Rice with Genome Editing Technologies

Chunxue Yan^1,2, Huicong Meng^1,2, Yanxin Pei^1,2, Wei Sun², Jinshan Zhang^1,2

¹Bellagen Biotechnology Co. Ltd, ²School of Life Sciences,Shandong Normal University

Journal (Bioengineering)

Designing sgRNA and Construction of Vector for Genome Editing to Breed Rice with Resistant Starch

Chunxue Yan^1,2, Huicong Meng^1,2, Yanxin Pei^1,2, Wei Sun², Jinshan Zhang^1,2

¹Bellagen Biotechnology Co. Ltd, ²School of Life Sciences,Shandong Normal University

Journal (Bioengineering)

Genotyping Transgenic Plants and Harvesting Seeds for Selecting Rice Varieties Enriched with Resistant Starch

Chunxue Yan^1,2, Huicong Meng^1,2, Yanxin Pei^1,2, Wei Sun², Jinshan Zhang^1,2

¹Bellagen Biotechnology Co. Ltd, ²School of Life Sciences,Shandong Normal University

Journal (Bioengineering)

Consistent Generation of Human Vascular Organoids Using Microwell Technology

Cong Xu*¹, Siyu He*¹, Yuefei Zhu¹, Gazelle Crasto², Caroline Chen¹, Morgan L. Clay³, Yeh-Hsing Lao³, Kam W. Leong¹

¹Department of Biomedical Engineering,Columbia University, ²College of Dental Medicine, Division of Periodontics,Columbia University, ³Department of Pharmaceutical Sciences, University at Buffalo,The State University of New York

Journal (Bioengineering)

Vascular Organoid Generation from Human-Induced Pluripotent Stem Cells

Cong Xu*¹, Siyu He*¹, Yuefei Zhu¹, Gazelle Crasto², Caroline Chen¹, Morgan L. Clay³, Yeh-Hsing Lao³, Kam W. Leong¹

Journal (Bioengineering)

Production and Optimization of LTE, a Leishmania tarentolae Derived Cell-Free Protein Expression System for Recombinant Protein Production

Wayne A. Johnston^1,2,3,4, Juan Alfaro Palma^1,2,3,4, Kirill Alexandrov^1,2,3,4,5

¹CSIRO-QUT Synthetic Biology Alliance,Queensland University of Technology, ²ARC Centre of Excellence in Synthetic Biology,Queensland University of Technology, ³Centre for Agriculture and the Bioeconomy,Queensland University of Technology, ⁴School of Biology and Environmental Science,Queensland University of Technology, ⁵Centre for Genomics and Personalised Health,Queensland University of Technology

Journal (Bioengineering)

Cell Seeding of Recombinant Spider Silk Membranes for Improved In Vitro Barrier Models

Linnea Gustafsson*^1,2, Savvini Gkouma*³, Alexander Jönsson⁴, Martin Dufva⁴, My Hedhammar³

¹Spiber Technologies AB, ²Division of Micro and Nanosystems,KTH Royal Institute of Technology, ³Division of Protein Technology,KTH Royal Institute of Technology, ⁴Department of Health Technology,Technical University of Denmark

Journal (Bioengineering)

Nanofibrillar Basement Membrane Mimic Made of Recombinant Functionalized Spider Silk in Custom-Made Tissue Culture Inserts

Linnea Gustafsson*^1,2, Savvini Gkouma*³, Alexander Jönsson⁴, Martin Dufva⁴, My Hedhammar³

Journal (Bioengineering)

Preparation of Hyaluronic Acid-Collagen Hydrogel in a Tissue Culture Insert for Replicating the Glioma Tumor Microenvironment

Samantha Howerton^1,2, Yanping Liang¹, Jennifer Hammel^1,3, Benjamin Purow⁴, Jennifer Munson^1,3

¹Fralin Biomedical Research Institute at Virginia Tech Carilion, ²Translational Biology, Medicine, and Health Graduate Program,Virginia Tech, ³Department of Biomedical Engineering & Mechanics,Virginia Tech, ⁴Department of Neurology,University of Virginia School of Medicine

Journal (Bioengineering)

A Photopolymerizable Hyaluronic Acid-Collagen Model of the Invasive Glioma Microenvironment with Interstitial Flow

Samantha Howerton^1,2, Yanping Liang¹, Jennifer Hammel^1,3, Benjamin Purow⁴, Jennifer Munson^1,3

Journal (Bioengineering)

Quantification of Adeno-Associated Viral Genomes in Purified Vector Samples by Digital Droplet Polymerase Chain Reaction

Nathalie Van den Berghe¹, Elien Costermans¹, Samir Nuseibeh¹, Tine Brouns¹, Inge Van Hove¹, Benjamien Moeyaert¹, Els Henckaerts¹

¹Trellis Research Group, Department of Cellular and Molecular Medicine, Department of Microbiology, Immunology and Transplantation,KU Leuven

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