Inducing Acute Pons Infarction in a Rat Model Using Electrical Stimulation

Abstract

Source: Luo, M., et al. Establishment of Acute Pontine Infarction in Rats by Electrical Stimulation. J. Vis. Exp. (2020)

This video demonstrates a procedure to induce tissue damage or infarction in the pons of an anesthetized rat using electrical stimulation.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Establishment of infarction in the pons

1. Preheat the heating pad immediately before anesthesia.
2. Attach the skull drill to the holder on the stereotaxic frame.
3. Intraperitoneally inject the rats with 50 mg/kg ketamine and 5 mg/kg xylazine. Check for the lack of toe-pinch response.
4. Mount the rat onto the stereotaxic frame in the prone position. Position the ear bars above the ear canal to secure the head. Ensure that the skull is kept horizo....

Results

Figure 1: Infarction establishment. (A) A hole made in the skull. (B) The sheath is moved to the hole. (C) Injection of the sheath. (D) Injection of the probe. (E) The an.......

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
4-0 sucture	Shanghai Jinzhong		Surgical instruments
Adhesive tape	Shanghai Jinzhong		Surgical instruments
Animal anesthesia system	RWD		Wear mask when using the system
Bone cement	Shanghai Jinzhong		Surgical instrument
Cured clamp	Shanghai Jinzhong		Surgical instrument
General tissue scissors	Shanghai Jinzhong		Surgical instrument
IndoPhors	Guoyao of China		Sterilization
Isoflurane	RWD	217181101
Lesion Making Device	Shanghai Yuyan		Making a lesion
MRI system	Bruker Biospin		Confirmation of infarction in vivo
Needle holder	Shanghai Jinzhong		Surgical instrument
Penicilin	Guoyao of China		Infection Prevention
Probe	Anke		Need some modification
Q-tips	Shanghai Jinzhong		Surgical instrument
Shearing scissors	Shanghai Jinzhong		Surgical instrument
Stereotaxic apparatus	RWD
Suture needle	Shanghai Jinzhong		Surgical instrument
Tissue holding forcepts	Shanghai Jinzhong		Surgical instrument
TTC	Sigma-Aldrich	BCBW5177	For infarction confirmation in vitro