This video demonstrates the procedure of noise-induced hearing loss in mice. High-volume sound damages hair cells in the inner ear and disrupts auditory pathways, resulting in hearing impairment.
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Method Article
Inducing Hearing Loss in Mice Through Prolonged High-Volume Sound Exposure
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April 28th, 2025
In This Article
Abstract
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Induction of noise-induced hearing loss (NIHL) in mice
- Prepare the cage for the experimental mice
- To do so, use a rat trap cage with dimensions of 14 cm × 17 cm × 24 cm. Cut four pie....
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Results
Figure 1: Voltage calculation from calibration. For each.......
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Materials
| Name | Company | Catalog Number | Comments |
|---|---|---|---|
| 1/4" CCP Free-field Standard Microphone Set | GRAS | 428158 | For noise exposure |
| Rat trap cage | 14 cm x 17 cm x 24cm | ||
| Sound amplifier, MT-1000 | unika | For noise exposure | |
| Sound generator/analyzer/miscellaneous, FW-02 | CLIO | 620300719 | For noise exposure |
| Soundproof chamber | IEA Electro-Acoustic Technology | For noise exposure and ABR | |
| Speaker | IEA Electro-Acoustic Technology | For noise exposure |
