Erratum: Visualizing RNA Localization in Xenopus Oocytes

10 juin 2011

Dans cet article

Résumé

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A correction to the step 2 of Part 1 for article Visualizing RNA Localization in Xenopus Oocytes.

Protocole

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A correction was made to Visualizing RNA Localization in Xenopus Oocytes. There was an error in step 2 of part 1. Some characters in the reagents table had the incorrect symbols. This was corrected to:

a. 10X Tx buffer (see M&M)2 μl
b. 20X cap/NTP mix (see M&M)1 μl
c. 1 mM Alexa Fluor 546-14-UTP (Invitrogen)1 μl
d. UTP, [α-32P](1 μCi/μl) (Perkin Elmer)1 μl
e. DEPC-H2O11 μl
f. 0.2 M DTT1 μl
g. RNasin (Promega)1 μl
h. linear template DNA1 μl
i. RNA Polymerase (Promega)1 μl

instead of:

a. 10' Tx buffer (see M&M)2 μl
20' cap/NTP mix (see M&M)1 μl
1 mM Alexa Fluor 546-14-UTP (Invitrogen)1 μl
UTP, [α-32P](1 μCi/μl) (Perkin Elmer)1 μl
a. DEPC-H2O11 μl
b. 0.2 M DTT1 μl
c. RNasin (Promega)1 μl
d. linear template DNA1 μl
e. RNA Polymerase (Promega)1 μl

Déclarations de divulgation

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No conflicts of interest declared.

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Mots-clés

Imagerie des ovocytesmicroscopie fluorescencehybridation in situd tection de l ARNd veloppement embryonnairebiologie mol culairebiologie cellulaire