Protocol
A correction was made to Visualizing RNA Localization in Xenopus Oocytes. There was an error in step 2 of part 1. Some characters in the reagents table had the incorrect symbols. This was corrected to:
| a. 10X Tx buffer (see M&M) | 2 μl |
| b. 20X cap/NTP mix (see M&M) | 1 μl |
| c. 1 mM Alexa Fluor 546-14-UTP (Invitrogen) | 1 μl |
| d. UTP, [α-32P](1 μCi/μl) (Perkin Elmer) | 1 μl |
| e. DEPC-H2O | 11 μl |
| f. 0.2 M DTT | 1 μl |
| g. RNasin (Promega) | 1 μl |
| h. linear template DNA | 1 μl |
| i. RNA Polymerase (Promega) | 1 μl |
instead of:
| a. 10' Tx buffer (see M&M) | 2 μl |
| 20' cap/NTP mix (see M&M) | 1 μl |
| 1 mM Alexa Fluor 546-14-UTP (Invitrogen) | 1 μl |
| UTP, [α-32P](1 μCi/μl) (Perkin Elmer) | 1 μl |
| a. DEPC-H2O | 11 μl |
| b. 0.2 M DTT | 1 μl |
| c. RNasin (Promega) | 1 μl |
| d. linear template DNA | 1 μl |
| e. RNA Polymerase (Promega) | 1 μl |
Disclosures
No conflicts of interest declared.
