Intestinal Tissue
Intestinal Tissue - Related Videos
08:45
Tissue Engineering of the Intestine in a Murine Model
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Cited by 29 •
2012
This article and the accompanying video present our protocol for generating tissue-engineered intestine in the mouse, using an organoid units-on-scaffold approach.
05:10
Three-dimensional Quantification of Intestinal Mucus Using Whole-mount Tissue Imaging
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2025
This study presents a 3D imaging method using whole-mount intestinal tissues and multi-photon microscopy to quantify secreted mucus, enabling precise volumetric analysis and visualization of mucus dynamics in response to stimuli like carbamoylcholine chloride.
01:45
Visualization of Amino Acid Metabolites in Intestinal Tissues of Infected Mice
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2026
Source: Specker, J. T., et al. Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection. J. Vis. Exp. (2022)This video demonstrates the use of matrix-assisted laser desorption/ionization (MALDI) mass spectrometry to visualize amino acid distribution in intestinal tissues from mice infected with Clostridioides difficile, alone or in combination with Enterococcus faecalis. The procedure involves tissue...
07:42
Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses
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Cited by 166 •
2016
Accurate identification and location of epithelial cells along the intestinal mucosal lining are essential to define different cell lineages. Proper imaging of intestinal tissues is crucial for identification of protein expression patterns with maximum resolution. This study aims to delineate the optimal methods and conditions for processing mouse intestinal tissues.
02:42
Quantification of Tissue Bacterial Load in a Mouse Model of Chronic Intestinal Infection
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2026
Source: Lo, B. C., et al. Chronic Salmonella Infection Induced Intestinal Fibrosis. J. Vis. Exp. (2019)The video demonstrates the quantification of bacterial load in a mouse model of chronic infection with streptomycin-resistant Salmonella typhimurium. Following euthanasia and sterile dissection, cecal and splenic tissues are harvested, homogenized with steel beads to release bacteria, and serially diluted. Dilutions are plated on selective agar, and visible colonies are counted to estimate...
FAQs
Successful intestinal tissue engineering depends on coordinating multiple cellular and structural elements rather than reproducing epithelial cells alone. Epithelial, stromal, immune, vascular, and neural components contribute to the tissue’s physiological complexity, while biomaterials and extracellular-matrix cues help organize cell behavior. This coordinated design supports models that more closely represent intestinal function and barrier protection.
Biomaterials and extracellular-matrix cues provide the surroundings needed for intestinal cells to attach, differentiate, and organize. Their function is not merely structural: these signals help guide cells toward tissue arrangements that include lumen-like structures. Incorporating such cues can therefore improve the biological organization of engineered constructs and make them more useful for studying intestinal processes.
Controlled culture conditions help maintain the signals required for cell attachment, differentiation, and tissue organization. In engineered intestinal systems, these conditions work together with biomaterials and extracellular-matrix cues to promote lumen-like architecture. Adjusting the culture environment is therefore central to obtaining reproducible models capable of supporting studies of epithelial transport, development, host-microbe interactions, or disease.
Organoid-based models provide an in vitro platform for examining intestinal development, epithelial transport, host-microbe interactions, and disease. Their value comes from offering a more physiologically complex setting than isolated cellular observations, while remaining accessible for laboratory investigation. In bioengineering, they complement engineered tissue approaches and support efforts to create more predictive systems for testing biological responses.
A general workflow combines intestinal cells with suitable biomaterials, extracellular-matrix cues, and controlled culture conditions. The culture environment is then used to promote cell attachment, differentiation, and formation of lumen-like structures. The resulting engineered tissue or organoid-based model can be examined for its usefulness in studying intestinal function, disease processes, epithelial transport, or interactions with microbes.
Researchers can use engineered intestinal tissue models when they need to investigate development, epithelial transport, host-microbe interactions, or disease in an organized in vitro system. These models also provide platforms for drug testing, allowing intestinal responses to be examined in a tissue-like context. Their broader purpose is to improve experimental relevance compared with less physiologically complex approaches.
Intestinal tissue engineering is relevant to regenerative medicine because it aims to produce organized, functional tissue rather than only isolated cells. The combination of cellular components, biomaterials, extracellular-matrix cues, and controlled culture conditions supports formation of tissue architecture and lumen-like structures. This work may contribute to the longer-term goal of developing functional tissue replacement while also improving in vitro research models.