Holographic Image Acquisition
Holographic Image Acquisition - Related Videos
09:12
Construction of a High Resolution Microscope with Conventional and Holographic Optical Trapping Capabilities
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Cited by 4 •
2013
The system described herein employs a traditional optical trap as well as an independent holographic optical trapping line, capable of creating and manipulating multiple traps. This allows for the creation of complex geometric arrangements of refractive particles while also permitting simultaneous high-speed, high-resolution measurements of the activity of biological enzymes.
03:25
Two-Photon Holographic Microscopy to Study Neural Activity and Connectivity in a Mouse Model
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2025
Source: Kato, D. et. al., Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy. J. Vis. Exp. (2022)This video demonstrates the two-photon imaging technique combined with optogenetics using holographic microscopy. Using a spatial light modulator, this technique enables precise neuronal stimulation expressing the red-light-sensitive protein and calcium imaging with minimal photodamage. This approach provides insights into the functional connectivity and...
07:38
Multimodal Quantitative Phase Imaging with Digital Holographic Microscopy Accurately Assesses Intestinal Inflammation and Epithelial Wound Healing
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Cited by 18 •
2016
Accurate assessment of anti-inflammatory effects is of utmost importance for the evaluation of potential new drugs for the treatment of inflammatory bowel disease. Digital holographic microscopy provides assessment of inflammation in murine and human colonic tissue samples as well as automated multimodal evaluation of epithelial wound healing in vitro.
10:16
Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
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Cited by 9 •
2014
The three-dimensional locations of weakly-scattering objects can be uniquely identified using digital inline holographic microscopy (DIHM), which involves a minor modification to a standard microscope. Our software uses a simple imaging heuristic coupled with Rayleigh-Sommerfeld back-propagation to yield the three-dimensional position and geometry of a microscopic phase object.
05:45
Uncovering Hidden Dynamics of Natural Photonic Structures Using Holographic Imaging
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Cited by 6 •
2022
The paper is primarily focused on the combined power of optical (linear and nonlinear) and holographic methods used to reveal phenomena at the nanoscale. The results obtained from the biophotonic and oscillatory chemical reactions' studies are given as representative examples, highlighting holography's ability to reveal dynamics at a nanoscale.
FAQs
The reference beam provides a known light field that interferes with light scattered by the sample at the camera. This interference pattern records information related to both the scattered light’s phase and amplitude, rather than intensity alone. Numerical reconstruction can then use that encoded information to generate images corresponding to different depths within the recorded object.
Phase information supports quantitative measurements of three-dimensional structure without requiring labels. In bioengineering studies, this enables researchers to examine cellular or tissue morphology while leaving the sample available for continued observation. The resulting measurements can therefore support analysis of changes such as growth, movement, and interactions over the course of an experiment.
Intensity-only imaging records brightness, whereas holographic image acquisition preserves additional information carried by the light field, including phase and amplitude. That distinction allows numerical reconstruction at multiple depths and provides quantitative three-dimensional information. For biological samples, the approach can reveal structural features of cells, microorganisms, and tissues without relying on labels described in the source context.
The process begins by directing coherent light toward the sample so that the sample scatters part of the illumination. The scattered light interferes with a reference beam on a camera, producing a hologram. Numerical algorithms subsequently reconstruct the recorded information at selected depths, after which researchers can examine three-dimensional structure and related biological measurements.
Reconstructed images can support quantitative assessment of cell morphology, growth, motility, and interactions. Because the method provides information across different depths, researchers can examine structural changes in three dimensions rather than relying only on a two-dimensional intensity image. These outcomes are useful for characterizing biological behavior while retaining the sample for further observation.
The approach is useful when researchers need label-free visualization of cells, microorganisms, or tissue structures and want to continue observing the same sample. Its quantitative three-dimensional measurements can contribute to diagnostic studies, tissue-engineering research, and broader biomedical investigations. In bioengineering, these capabilities connect image-based structural analysis with studies of growth, motility, and cellular interactions.