On-chip Photopolymerization
On-chip Photopolymerization - Related Videos
07:50
Chip-Based Digital PCR to Detect Rare Transcript Variants Using a Nanofluidic Chip
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2025
This video demonstrates chip-based digital PCR — a variation of the digital PCR technique that is useful in detecting rare transcript variants. The PCR reaction is partitioned into the chambers of a nanofluidic chip, each of which acts as an independent reaction. The detection of fluorescence signals from the chambers with amplified targets confirms the presence of rare transcript variants in the sample.
01:35
Organ-on-Chip Models
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2026
Introduction to Organ-on-Chip Models Organ-on-chip models are advanced microphysiological systems that recreate key structural and functional features of human organs in a controlled laboratory setting. These devices integrate living human cells within microfluidic channels, allowing researchers to simulate tissue–tissue interfaces, fluid flow, and mechanical forces that are essential for normal organ function. Unlike traditional cell cultures, which often lack these dynamic physiological cues,...
11:23
Lensless Fluorescent Microscopy on a Chip
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Cited by 8 •
2011
A lensless on-chip fluorescent microscopy platform is demonstrated that can image fluorescent objects over an ultra-wide field-of-view of e.g., >0.6-8 cm2 with <4μm resolution using a compressive sampling based decoding algorithm. Such a compact and wide-field fluorescent on-chip imaging modality could be valuable for high-throughput cytometry, rare-cell research and microarray-analysis.
12:43
On-Chip Endothelial Inflammatory Phenotyping
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Cited by 3 •
2012
Microfluidic flow chambers etched by photolithography and fabricated from PDMS are applied to probe functional outcomes associated with EC dysfunction and inflammation. In a representative experiment, the ability of differential shear stress to modulate monocytic cell adhesion to cytokine activated EC monolayers is demonstrated.
11:16
A Microfluidic Chip for ICPMS Sample Introduction
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Cited by 4 •
2015
We present a discrete droplet sample introduction system for inductively coupled plasma mass spectrometry (ICPMS). It is based on a cheap and disposable microfluidic chip that generates highly monodisperse droplets in a size range of 40−60 µm at frequencies from 90 to 7,000 Hz.
FAQs
The key control is selective conversion: patterned illumination activates polymerization only in chosen regions, while surrounding precursor remains fluid or removable. This creates boundaries with microscale precision and allows geometry to be adjusted through the light pattern and exposure timing. Such control is valuable when a device must combine solid structures with open channels or defined biological spaces.
The photoreactive liquid resin or hydrogel precursor serves as the starting material that changes phase after illumination. A resin can support solid device features, whereas a hydrogel precursor can support hydrated constructs, including cell-laden hydrogels. This distinction lets bioengineers match the fabricated material to its intended role, such as a scaffold, tissue-engineering construct, or fluidic component.
Because structures form directly within the chip, biomaterials and fluidic components can be positioned in controlled geometries on the same small-scale platform. This arrangement is especially relevant to organ-on-chip systems and biosensors, where engineered materials, fluid handling, and analytical functions must operate together. The approach therefore supports closer integration between device architecture and biological experimentation.
A basic workflow begins by positioning a photoreactive resin or hydrogel precursor in the microfluidic device, followed by exposure through a selected light pattern. Illuminated regions polymerize into the intended structure, while unexposed material remains removable or fluid. Removing or retaining those regions as appropriate produces the desired scaffold, construct, or integrated fluidic feature.
Pattern geometry and the timing of illumination are central process variables because they determine where and when conversion occurs. A patterned exposure can define the shape of a scaffold, hydrogel, or fluidic feature, while temporal control helps coordinate fabrication with the surrounding chip environment. These variables therefore influence spatial precision and integration with living-cell or analytical components.
In bioengineering, the approach is useful when researchers need microscale structures that connect materials, cells, and fluid handling. Reported uses include microscale scaffolds, tissue-engineering constructs, cell-laden hydrogels, fluidic components, organ-on-chip systems, biosensors, and regenerative medicine research. The resulting geometrical control can support studies of how engineered environments interact with biological systems.