Drug-resistant Bacteria
Drug-resistant Bacteria - Related Videos
14:04
Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
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Cited by 17 •
2013
Lytic phage biosensors and antibody beads are able to discriminate between methicillin resistant (MRSA) and sensitive staphylococcus bacteria. The phages were immobilized by a Langmuir-Blodgett method onto a surface of a quartz crystal microbalance sensor and worked as broad range staphylococcus probes. Antibody beads recognize MRSA.
02:45
Bacterial Growth on a Microfluidic Chip to Study Antibiotic Drug Resistance
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2025
Source: Liu, P. C., et al. Design and Use of a Low Cost, Automated Morbidostat for Adaptive Evolution of Bacteria Under Antibiotic Drug Selection. J. Vis. Exp. (2016)This video demonstrates the use of a microfluidic chip to visualize and compare the growth and morphology of wild-type and trimethoprim-resistant E. coli strains under trimethoprim antibiotic stress.
11:56
Nanomechanics of Drug-target Interactions and Antibacterial Resistance Detection
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Cited by 4 •
2013
Acquired resistance to antibiotics is a major public healthcare problem and is presently ranked by the WHO as one of the greatest threats to human life. Here we describe the use of cantilever technology to quantify antibacterial resistance, critical to the discovery of novel and powerful agents against multidrug resistant bacteria.
03:16
Isolation of Antibiotic-Resistant Bacteria from a Water Sample for Molecular Analysis
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2025
Source: Ghadigaonkar, D. and Rath, A. Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes. J. Vis. Exp. (2023)This video demonstrates the isolation and preparation of antibiotic-resistant bacteria from a water sample using selective media. The DNA is extracted from antibiotic-resistant bacteria and amplified to identify the bacterial antibiotic-resistant genes.
08:46
Implementation of In Vitro Drug Resistance Assays: Maximizing the Potential for Uncovering Clinically Relevant Resistance Mechanisms
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Cited by 2 •
2015
Drug resistance to targeted therapeutics is widespread and the need to identify mechanisms of resistance--prior to or following clinical onset--is critical for guiding alternative clinical management strategies. Here, we present a protocol to couple derivation of drug-resistant lines in vitro with sequencing to expedite discovery of these mechanisms.
FAQs
Mutations can change bacterial features that antibiotics depend on for activity. In the context of drug-resistant bacteria, these changes may modify an antibiotic’s target, reduce cell permeability, or increase removal of the drug through efflux pumps. Studying the resulting mechanisms helps researchers explain why a treatment may lose effectiveness and identify strategies for developing alternative therapies.
Horizontal gene transfer allows bacteria to acquire resistance-related genetic material from other bacteria rather than relying only on mutations arising within their own cells. This process can accelerate the appearance and spread of resistance traits. Understanding it is therefore important for genomic surveillance, because researchers need to track how resistance mechanisms move through bacterial populations.
These mechanisms interfere with different stages of antibiotic action. A changed target can prevent the drug from acting effectively, reduced cell permeability can limit how much drug enters the bacterium, and efflux pumps can remove antibiotic molecules from the cell. Distinguishing among these mechanisms supports interpretation of resistance patterns and guides research into treatments that can overcome them.
Resistance can lead to treatment failure because an antibiotic that would normally inhibit or kill susceptible bacteria may no longer work against a resistant strain. The resulting challenge extends beyond an individual infection: difficult-to-treat bacteria can continue contributing to transmission and disease. This connection makes resistance research relevant to both clinical decision-making and broader public health planning.
Antimicrobial susceptibility testing evaluates how bacterial strains respond to antibiotics. Its results help identify whether a strain is likely to be inhibited by a particular drug and can reveal patterns consistent with resistance. In clinical and research settings, this information supports assessment of treatment options, investigation of treatment failure, and monitoring of changing resistance across bacterial populations.
Genomic surveillance examines bacterial genetic information to monitor resistance-related changes and the distribution of resistance traits. It can support investigation of how resistance emerges and spreads, especially when genetic mechanisms or horizontal gene transfer are involved. These findings help researchers follow trends, recognize important resistance patterns, and inform strategies intended to limit transmission.
Antibiotic stewardship is used to promote more responsible antibiotic use and reduce pressures that contribute to resistance. It complements laboratory testing and surveillance by connecting evidence about bacterial susceptibility and spread with treatment practices. In biology and clinical research, stewardship helps support strategies aimed at reducing treatment failure, transmission, and the wider public health burden of difficult-to-treat infections.
Mechanistic research identifies how bacteria evade antibiotic activity, including target alteration, reduced permeability, drug inactivation, and efflux. Researchers can use that information to evaluate new drugs or treatment strategies designed to address these obstacles. Linking mechanism studies with susceptibility testing and genomic surveillance improves understanding of which approaches may be useful against challenging bacterial infections.