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Chapters
- 00:05Title
- 02:08Gold-immobilized Phage
- 02:52Testing of Immobilized Phage Infectivity on a Dry Surface
- 03:23Testing of Lytic Activity of Free and Bound Phage in Liquid
- 04:13Phage Spheroids Preparation
- 05:01Biosensor Preparation
- 06:47Biosensor Testing, Discrimination of Methicillin-Sensitive (MSSA) and -Resistant (MRSA) Staphylococcus Aureus Bacteria
- 08:17Results: Representative Biosensor Detection of Antibiotic Resistant Staphylococcus Bacteria
- 13:20Conclusion
Lytic phage biosensors and antibody beads are able to discriminate between methicillin resistant (MRSA) and sensitive staphylococcus bacteria. The phages were immobilized by a Langmuir-Blodgett method onto a surface of a quartz crystal microbalance sensor and worked as broad range staphylococcus probes. Antibody beads recognize MRSA.
