Live Embryo Injection
Live Embryo Injection - Related Videos
05:51
Microinjection of Live Drosophila Embryos: Early Delivery of Reagents to the Developing Embryo
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2023
This video describes how to perform microinjections that deliver reagents into live Drosophila embryos. Researchers can use this method to introduce exogenous compounds like nucleic acids and proteins during the early stages of embryo development. The featured protocol demonstrates how to set up and perform the procedure for injection of any soluble reagent–here, a fluorescent protein for live imaging experiments.
06:10
Injection of Microbubbles into Isolated Mouse Embryos: A Technique to Deliver Microbubble Contrast Agents into the Vasculature of Living Murine Embryos
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2025
This video demonstrates a procedure to inject microbubble contrast agents into an isolated late gestation stage mouse embryo. The microbubble contrast agent-based ultrasound imaging helps visualize and characterize the vascular environment of the embryo.
09:10
Two-Photon-Based Photoactivation in Live Zebrafish Embryos
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Cited by 5 •
2010
Multiphoton microscopy allows control of low energy photons with deep optical penetration and reduced phototoxicity. We describe the use of this technology for live cell labeling in zebrafish embryos. This protocol can be readily adapted for photo-induction of various light-responsive molecules.
08:30
RNAi Interference by dsRNA Injection into Drosophila Embryos
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Cited by 6 •
2011
RNA interference has been proven very effective to analyze gene function in Drosophila tracheal development. A detailed protocol used by Jiang lab to inject dsRNA into fly embryos to knockdown gene expression is illustrated. This technique has the potential for screening genes required for tissue and organ development in Drosophila.
10:10
Observing Mitotic Division and Dynamics in a Live Zebrafish Embryo
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Cited by 8 •
2016
Mitosis is critical to every living organism and defects often lead to cancer and developmental disorders. Using this imaging protocol and zebrafish as a model system, researchers can visualize mitosis in a live vertebrate organism and the multitude of defects that arise when mitotic processes are defective.
FAQs
Targeting the cytoplasm, nucleus, or another defined compartment can change what the experiment is designed to examine. The selected location determines where the introduced material is deposited during development, while the controlled volume helps limit variation between embryos. This spatial precision makes Live Embryo Injection useful for linking molecular interventions with developmental outcomes.
Embryo viability is central because the intervention must occur without ending development before biological effects can be observed. The technique therefore combines microsurgical penetration with delivery of a controlled amount of material. Maintaining a living embryo preserves the developmental context needed to study cell lineage, embryonic patterning, and the processes that shape development.
The introduced substance helps define the experimental question. Genetic material can support studies of gene function or, with appropriate reagents, transgenesis and genome editing. Proteins and drugs can be used to examine how molecular signals influence development. The resulting embryos connect a selected molecular input with observable developmental processes.
A basic workflow begins with viewing the living embryo under a microscope, positioning a fine glass micropipette, penetrating the embryo, and depositing a controlled volume in the cytoplasm, nucleus, or another defined compartment. The embryo then serves as the biological system in which the introduced material can be related to developmental observations.
The microscope provides the visual setting for precise microsurgery, while the fine glass micropipette provides the route for delivery. Together, these components allow material to be placed in a selected embryonic compartment rather than introduced without spatial control. This arrangement is important when location and amount both matter to interpreting developmental effects.
Live Embryo Injection supports several biology applications, including testing gene function, tracing cell lineage, examining embryonic patterning, and studying developmental processes. When suitable reagents are delivered, it can also enable transgenesis or genome editing. These applications make injected embryos direct models for investigating how molecular signals shape development and disease.