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Two-Photon-Based Photoactivation in Live Zebrafish Embryos
JoVE Journal
Biology
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JoVE Journal Biology
Two-Photon-Based Photoactivation in Live Zebrafish Embryos

Two-Photon-Based Photoactivation in Live Zebrafish Embryos

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09:10 min

December 24, 2010

DOI:

09:10 min
December 24, 2010

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Summary

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Multiphoton microscopy allows control of low energy photons with deep optical penetration and reduced phototoxicity. We describe the use of this technology for live cell labeling in zebrafish embryos. This protocol can be readily adapted for photo-induction of various light-responsive molecules.

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