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JoVE Journal
Biology

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Two-Photon-Based Photoactivation in Live Zebrafish Embryos
 

Two-Photon-Based Photoactivation in Live Zebrafish Embryos

Article DOI: 10.3791/1902
December 24th, 2010

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Summary December 24th, 2010

Multiphoton microscopy allows control of low energy photons with deep optical penetration and reduced phototoxicity. We describe the use of this technology for live cell labeling in zebrafish embryos. This protocol can be readily adapted for photo-induction of various light-responsive molecules.

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