Lumen Formation
Lumen Formation - Related Videos
03:21
Localized Delivery of Bacteria into the Intestinal Lumen of a Zebrafish Larva Using Microinjection
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2025
Source: Li, J. et al. Development of a Larval Zebrafish Infection Model for Clostridioides difficile. J. Vis. Exp. (2020)This video demonstrates the microinjection-based delivery of fluorescently labeled Clostridioides difficile into the intestinal lumen of an anesthetized zebrafish larva. It outlines the steps involved in larval preparation, bacterial injection, and fluorescence microscopy–based confirmation of bacterial localization within the intestine.
02:34
Quantification of Group B Streptococcus Load in the Mouse Vaginal Lumen via Swabbing and Chromogenic Culture
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2026
Source: Patras, K. A. & Doran, K. S. A Murine Model of Group B Streptococcus Vaginal Colonization. J. Vis. Exp. (2016)This video demonstrates the quantification of vaginal Group B Streptococcus (GBS) load in a mouse model using swabbing and chromogenic culture. It outlines the steps involved in sample collection, plating, and colony counting to quantify GBS load in the mouse vaginal lumen.
03:36
Gold Immunostaining of Exosomal Sections: A Technique to Visualize Diagnostic Markers in Exosomal Lumen
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2023
In this video, we demonstrate gold immunostaining of biomarkers localized within the lumen region of ultrathin exosomal sections. This technique enables early diagnosis of diseases by visualization of target molecules within the exosomes secreted by the diseased cells.
03:21
Gut Acidification Monitoring Assay: A Technique to Study the Acidification of the Intestinal Lumen in Drosophila using Bromophenol Blue Dye
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2025
This video demonstrates an assay to monitor gut acidification in Drosophila flies. After the ingestion of food supplemented with a pH indicator dye, the acidification of the intestinal lumen results in a change in the color of the dye. Visual inspection of the digestive tract of the flies to examine the color change provides evidence of acidification.
02:16
Solution Formation
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2020
There is no one solvent that can dissolve every type of solute. Some substances that readily dissolve in a certain solvent might be insoluble in a different solvent. A simple way to predict which substances dissolve in which solvent is the phrase "like dissolves like". This means that polar substances, such as salt and sugar, dissolve in a polar substance like water. In contrast, non-polar substances are more soluble in non-polar solvents such as carbon tetrachloride. This selective solubility...
FAQs
Apical-basal polarity gives epithelial cells an organized orientation, while cell-cell adhesion and junctions keep neighboring cells coordinated. Together, these features help position the internal surface toward the developing space and preserve tissue architecture as cells arrange. If polarity or junctional organization becomes disrupted, the resulting structure can lose the regular organization expected of a healthy epithelial tissue.
Two complementary processes can contribute to the developing cavity. Cells may direct fluid transport toward the center, increasing the internal space, while centrally located cells may be removed to open it. The relative contribution of these mechanisms can vary, but both connect cellular organization with the emergence of a clear internal cavity in epithelial models.
These altered patterns can signal that epithelial polarity and tissue organization have been disrupted. In cancer research, researchers examine them as architectural readouts rather than treating lumen shape as an isolated feature. Comparing regular structures with irregular, filled, or multiple lumens helps investigate how tumor cells lose organization and develop behaviors associated with invasion into surrounding tissue.
Three-dimensional cultures and organoids provide a setting in which epithelial cells can organize around an internal space, allowing researchers to observe tissue architecture in a model system. These models make lumen-related changes visible during studies of tumor development. They also allow investigators to compare organized lumens with abnormal structures while examining how cancer alters epithelial organization.
A filled lumen is not simply a change in cavity appearance; it can be a sign of disrupted epithelial polarity and organization. In a cancer model, researchers can compare filled structures with more regularly organized lumens to examine changes in tissue architecture. This comparison supports investigation of tumor disorganization without treating one morphological feature as a complete explanation of disease behavior.
Because three-dimensional cultures and organoids reproduce aspects of epithelial architecture, researchers can use lumen organization as an outcome when examining treatment response. They can assess whether a model retains an organized lumen or displays abnormal structures such as filled or multiple lumens. This architectural readout complements studies of tumor development by showing how treatment relates to tissue organization.