Rat Kidney Perfusion
Rat Kidney Perfusion - Related Videos
10:42
Normothermic Machine Perfusion of Rat Kidneys for Transplantation
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2026
This protocol describes the ex vivo normothermic machine perfusion of a rat kidney for subsequent transplantation into a recipient rat.
08:19
The Mouse Isolated Perfused Kidney Technique
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Cited by 24 •
2016
The mouse isolated perfused kidney (MIPK) is a technique for keeping a mouse kidney under ex vivo conditions perfused and functional for 1 hr. The buffers and surgical technique are described in detail.
08:12
Normothermic Ex Vivo Kidney Perfusion for the Preservation of Kidney Grafts prior to Transplantation
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Cited by 43 •
2015
The severe organ shortage has resulted in increased use of marginal kidney grafts for transplantation. This has triggered interest in alternative storage methods, since marginal grafts especially tolerate cold storage poorly. The technique of normothermic ex vivo kidney perfusion (NEVKP) represents a novel preservation method for kidney grafts prior to transplantation.
03:30
Preparation of Decellularized Rat Kidney: A Procedure to Generate Acellular Vascularized Whole-organ Scaffold from a Harvested Rat Kidney
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2023
This video describes the preparation of a decellularized rat kidney. The obtained acellular vascular scaffold can serve as a promising platform for tissue engineering of organ grafts.
07:29
Intravascular Delivery of Biologics to the Rat Kidney
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Cited by 1 •
2016
The administration of drugs for recovery of kidney function requires control of the localization and distribution of the therapeutic compound. Here, we describe in detail a simple technique for intrarenal delivery of drugs in rats. This procedure may be easily performed with no mortality and high reproducibility.
FAQs
Regulated pressure and flow help maintain the vascular conditions needed for the isolated kidney to function during the experiment. These variables determine how the perfusate moves through the renal vasculature and therefore influence filtration and vascular transport. Controlling them also lets investigators examine responses to altered vascular conditions in a defined, reproducible setting.
An oxygenated, nutrient-containing perfusate supplies the isolated tissue with the environmental inputs specified for maintaining renal activity during a defined experimental period. This support is important because the model is intended to preserve measurable filtration, vascular transport, and cellular signaling rather than merely expose tissue to a test compound. Perfusate composition therefore affects how responses can be interpreted.
Because the preparation preserves aspects of filtration, vascular transport, and cellular signaling, investigators can assess how kidney tissue responds under controlled experimental conditions. These readouts connect vascular delivery with tissue-level responses and provide a way to study renal function outside the whole-animal environment. The model is therefore suited to mechanistic questions about kidney responses.
It allows an anticancer compound or tumor-associated signal to be evaluated in direct relation to kidney tissue and its perfused vasculature. Researchers can examine how altered vascular conditions affect delivery and how the tissue responds during a defined period. This makes the preparation useful for studying kidney-specific transport or response questions in a controlled ex vivo setting.
The core workflow begins by isolating the rat kidney and cannulating its renal vasculature. Investigators then circulate an oxygenated, nutrient-containing perfusate while regulating pressure and flow for the chosen experimental period. Once the preparation is functioning under these controlled conditions, they can introduce the experimental compound, signal, or vascular condition and assess resulting renal or tissue responses.
Pressure, flow, and the composition of the perfusate are central experimental variables. The solution provides oxygen and nutrients, while circulation remains regulated through the cannulated renal vasculature. Holding these features under defined conditions helps researchers attribute observed changes to the anticancer compound, tumor-associated signal, or altered vascular condition being studied rather than to uncontrolled delivery conditions.
It is particularly useful when the research question concerns kidney-specific effects of cancer-related interventions. Applications include evaluating anticancer compounds, tumor-associated signals, renal toxicity, therapeutic development, and drug delivery under altered vascular conditions. Its controlled ex vivo setting also supports mechanistic studies, allowing these questions to be examined during a defined experimental period.