Glucocorticoid Receptor Assay
Glucocorticoid Receptor Assay - Related Videos
08:02
Fecal Glucocorticoid Analysis: Non-invasive Adrenal Monitoring in Equids
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Cited by 6 •
2016
Adrenal activity can be assessed in the equine species by analysis of feces for corticosterone metabolites. The method offers a non-invasive option to assess long term patterns in both domestic and free ranging horses. This protocol describes the enzyme linked immunoassay involved and the associated biochemical validation.
11:49
Characterization of G Protein-coupled Receptors by a Fluorescence-based Calcium Mobilization Assay
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Cited by 26 •
2014
The here described fluorescence-based calcium mobilization assay is a medium-throughput reverse pharmacology screening system for the identification of functionally activating ligand(s) of orphan G protein-coupled receptors (GPCRs).
06:27
Competitive Binding Assay to Identify Compounds Disrupting Receptor-Ligand Interactions
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2025
In this video, we describe a flow cytometry-based competitive binding assay to detect the interactions between the CXC Chemokine Receptor 4 (CXCR4) and its fluorescently-labeled natural ligand CXC Chemokine Ligand 12 (CXCL12), in the presence of a CXCR4-targeting small molecule. Incubating CXCR4-expressing cells with lower small molecule concentrations allows CXCR4-CXCL12 binding to some extent, which progressively declines upon a gradual increase in small molecule concentrations.
11:07
Biochemical Reconstitution of Steroid Receptor•Hsp90 Protein Complexes and Reactivation of Ligand Binding
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Cited by 1 •
2011
An in vitro method for preparing functional glucocorticoid receptor (GR)•hsp90 protein complexes from purified proteins and cellular lysates is described. The method utilizes immunoadsorption of recombinant GR followed by salt-stripping and protein complex reconstitution. The importance of cofactors and buffer conditions are discussed, as are potential method applications.
04:55
Assaying Surface Expression of Chemosensory Receptors in Heterologous Cells
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Cited by 6 •
2011
Here we demonstrate a protocol to carry out live cell staining that can be used to detect odorant receptors on the surface of HEK293T cells conveniently. In addition, it may also be used to assay for surface expression of other chemosensory receptors or GPCRs.
FAQs
The key measurement is the receptor-linked response produced after exposure to a test substance. A substance may bind the receptor or alter its activity, and the assay converts that change into a quantifiable biochemical or reporter signal. This connects chemical exposure with a hormone-responsive signaling event relevant to stress-related gene regulation.
A biochemical format focuses on receptor interaction in a laboratory system, whereas a cell-based reporter format records receptor-related activity through a signal generated in cells. The choice affects how the response is represented, but both provide a quantitative endpoint for comparing test substances or environmental samples.
Chemical analysis and receptor activity provide complementary information. Chemical analysis can be paired with the assay when investigators evaluate water, sediments, consumer products, or other samples, while the receptor response indicates whether the tested material produces glucocorticoid-like activity. Together, these approaches support contaminant prioritization rather than relying on a single type of evidence.
An environmental screening workflow begins with a selected sample or test substance, followed by exposure of the receptor system or reporter cells. Investigators then quantify the resulting biochemical or cellular signal and compare the response across tested materials. Chemical analysis and ecological studies can add context when interpreting the screening result.
Applications can include water, sediments, consumer products, and other environmental or related samples identified for testing. The assay is useful when researchers need to screen many materials for glucocorticoid-like activity, helping them decide which contaminants or sample types warrant closer chemical characterization or ecological investigation.
A detected receptor response indicates glucocorticoid-like activity in the tested material, but it does not by itself replace chemical analysis or ecological studies. Environmental researchers can use the result as a screening signal for endocrine-disruption assessment and contaminant prioritization, then consider the finding with other evidence about potential biological and ecological effects.