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JoVE Journal
Biology

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Characterization of G Protein-coupled Receptors by a Fluorescence-based Calcium Mobilization Assay
 

Characterization of G Protein-coupled Receptors by a Fluorescence-based Calcium Mobilization Assay

Article DOI: 10.3791/51516
July 28th, 2014

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Summary July 28th, 2014

The here described fluorescence-based calcium mobilization assay is a medium-throughput reverse pharmacology screening system for the identification of functionally activating ligand(s) of orphan G protein-coupled receptors (GPCRs).

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