Micro-aspirator Assembly
Micro-aspirator Assembly - Related Videos
09:50
Single Cell Micro-aspiration as an Alternative Strategy to Fluorescence-activated Cell Sorting for Giant Virus Mixture Separation
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Cited by 5 •
2019
Here, we describe a single cell micro-aspiration method for the separation of infected amoebae. In order to separate viral subpopulations in Vermamoeba vermiformis infected by Faustoviruses and unknown giant viruses, we developed the protocol detailed below and demonstrated its ability to separate two low-abundance novel giant viruses.
14:19
Micro-drive Array for Chronic in vivo Recording: Tetrode Assembly
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Cited by 111 •
2009
In this protocol we demonstrate how to fabricate and condition tetrodes for use with a micro-drive array, which was designed for chronic electrophysiological recordings in rats. In addition, we illustrate the final stages of micro-drive array construction, which includes installing ground wires and a protective cone.
06:43
Fluid Aspiration from Mouse Glomeruli: A Method to Aspirate Glomerular Fluid from Mouse Using Two-Photon Mediated Micropuncture
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2023
This video showcases fluid aspiration from mouse glomeruli using the two-photon mediated micropuncture technique. The technique provides access to the living mouse’s glomeruli and thus helps gain insights into renal physiology.
07:10
Femoral Bone Marrow Aspiration in Live Mice
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Cited by 18 •
2014
This protocol describes a procedure for serial sampling of femoral bone marrow (BM) without requiring the sacrifice of mice. This procedure facilitates longitudinal studies of the BM composition of mice over time and provides serial access to cells within the BM for ex vivo and transplantation studies.
11:36
Assembly, Loading, and Alignment of an Analytical Ultracentrifuge Sample Cell
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Cited by 21 •
2009
The analytical ultracentrifuge (AUC) sample cell holds sample and reference buffer and during experiments and is exposed to high vacuum and rotor speeds up to 60,000 rpm. This video will demonstrate the rigorous attention to detail necessary for assembly, loading and alignment of this very important component of an AUC experiment.
FAQs
Leak-free connections preserve the relationship between pressure changes and movement of material through the fine tip. If air escapes at tubing joints or the holder, suction becomes less predictable, making it harder to collect, transfer, or position small biological samples accurately. In genetic micromanipulation, stable pressure control supports reproducible handling and helps reduce sample loss during embryo, cell, or nucleus manipulation.
The pressure source supplies the changes that drive aspiration, so its response directly affects how material moves into the fine capillary. Applying pressure changes in a controlled manner allows an operator to position or transfer small samples rather than relying on uncontrolled movement. This control is valuable when handling embryos, individual cells, nuclei, or other genetic material whose loss can compromise subsequent analysis.
The flexible tubing creates the connection between the pressure source and the pipette holder, while the holder supports the fine glass capillary or micropipette. Together, these parts maintain a continuous path for pressure transmission and support consistent manipulation at the working tip. Their condition therefore affects handling precision, sample loss, and reproducibility during micromanipulation procedures.
Assembly begins by linking the pressure source to flexible tubing, attaching the tubing to a pipette holder, and securing a fine glass capillary or micropipette at the working end. Before use, the connections should be checked for leaks and the system’s response to pressure changes. This preparation establishes the controlled suction needed for collection, transfer, or positioning.
In genetics, this setup is useful when a procedure requires manipulation of embryos, individual cells, nuclei, or other biological material at small volume. The operator can draw a sample into the capillary, move it, or position it without direct contact. These capabilities support cell isolation, experimental transfers, and downstream genetic analysis while limiting handling-related sample loss.
Reproducibility depends on performing similar collection, transfer, and positioning actions across samples. A properly assembled system makes pressure-driven handling more controlled, while leak-free connections reduce unpredictable changes in suction. As a result, researchers can more consistently isolate cells or nuclei, manipulate embryos, and prepare material for genetic analysis, with less risk of losing the sample during handling.