Metabolic Inhibitors
Metabolic Inhibitors - Related Videos
05:41
Metabolic Profile Analysis of Zebrafish Embryos
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Cited by 42 •
2013
Zebrafish represent a powerful vertebrate model that has been under-utilised for metabolic studies. Here we describe a rapid way to measure the in vivo metabolic profile of developing zebrafish that allows the comparison of different mitochondrial function parameters between genetically or pharmacologically manipulated embryos, thereby increasing the applicability of this organism.
01:52
Eukaryotic Transcription Inhibitors
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2020
Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells. Eukaryotic transcription inhibitors usually contain two distinct domains, a DNA...
06:59
Characterization of Metabolic Status in Nonhuman Primates with the Intravenous Glucose Tolerance Test
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Cited by 7 •
2016
The goal of this protocol is to present a standard method to perform intravenous glucose tolerance tests (IVGTTs) to assess glycemic control in nonhuman primates and assess their metabolic status from healthy to dysmetabolic.
08:45
Assessing Cellular Target Engagement by SHP2 (PTPN11) Phosphatase Inhibitors
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Cited by 4 •
2020
The ability to assess target engagement by candidate inhibitors in intact cells is crucial for drug discovery. This protocol describes a 384 well format cellular thermal shift assay that reliably detects cellular target engagement of inhibitors targeting either wild-type SHP2 or its oncogenic variants.
10:19
An Introduction to Cell Metabolism
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2023
In cells, critical molecules are either built by joining together individual units like amino acids or nucleotides, or broken down into smaller components. Respectively, the reactions responsible for this are referred to as anabolic and catabolic. These reactions require or produce energy typically in the form of a “high-energy” molecule called ATP. Together, these processes make up “Cell Metabolism,” and are hallmarks of healthy, living cells.JoVE’s introduction to cell metabolism briefly...
FAQs
The outcome depends on where and how the compound interferes with metabolism. Active-site binding can directly block enzyme function, whereas substrate competition reduces pathway activity by preventing normal substrate use. Inhibiting a transporter instead limits nutrient uptake before intracellular reactions occur. These distinctions help researchers connect a metabolic change with a particular enzyme, substrate, or transport process.
Metabolite levels reveal how pathway activity responds to inhibition. Accumulation or depletion of particular metabolites can indicate whether an enzymatic step, nutrient-uptake process, or downstream biosynthetic reaction has been affected. In infection and immune studies, these measurements help relate metabolic disruption to reduced energy generation, altered precursor availability, or changes in cellular function.
Researchers can examine how inhibition affects immune-cell activation, proliferation, and antimicrobial function. If blocking a metabolic process changes one of these responses, that pathway may support the corresponding cellular activity. Comparing effects across immune functions is useful because metabolic requirements can differ between resting and activated cells, allowing investigators to connect pathway activity with specific immune behaviors.
Their value comes from comparing how the pathogen and host respond to disruption of the same or related metabolic requirements. An inhibitor that restricts pathogen growth or function while producing a different effect in immune cells can help identify a potential pathogen-focused vulnerability. Conversely, shared effects highlight metabolic overlap that may influence host responses during infection.
A study can begin by selecting a metabolic component suspected to support pathogen activity or an immune response, then applying an inhibitor and examining changes in pathway activity, metabolite levels, or cellular function. Researchers can compare pathogen-related and immune-cell outcomes to determine whether the targeted process contributes primarily to infection, host defense, or both.
They are useful when researchers need functional evidence that a metabolic enzyme, transporter, or related component contributes to infection or immune activity. Suppressing the selected process and observing a corresponding change in pathogen behavior, immune-cell function, or infection outcome supports its biological importance. This approach helps prioritize metabolic processes for further therapeutic investigation.