Tcr Transgenic Models
Tcr Transgenic Models - Related Videos
19:05
Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay
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Cited by 9 •
2015
This manuscript describes how to conduct (single molecule) Förster Resonance Energy Transfer (FRET)- based assays to measure the binding dynamics between T-cell antigen receptor (TCR) and antigenic peptide-loaded MHC molecules as they occur within the immunological synapse of a T-cell in contact with a functionalized planar supported lipid bilayer.
13:59
Assaying β-amyloid Toxicity using a Transgenic C. elegans Model
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Cited by 76 •
2010
The intensely studied nematode worm Caenorhabditis elegans can be transgenically engineered to express the human β-amyloid peptide (Aβ). Induced expression of Aβ in C. elegans muscle leads to a rapid, reproducible paralysis phenotype that can be used to monitor treatments that modulate Aβ toxicity.
07:34
Induction of Intestinal Inflammation by Adoptive Transfer of CBir1 TCR Transgenic CD4+ T Cells to Immunodeficient Mice
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Cited by 9 •
2021
In this protocol, a gut microbiota antigen-specific T cell adoptive transfer colitis model is described. CD4+ T cells are isolated from CBir1 TCR transgenic mice. These are specific for an immunodominant gut microbiota antigen CBir1 flagellin, which is transferred into recipient Rag1-/- mice, leading to intestinal inflammation.
11:21
Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs
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Cited by 3 •
2017
The current protocol combines single cell paired human TCR alpha and beta chain sequencing with streamlined generation of retroviral vectors compatible with in vitro and in vivo TCR expression.
16:10
A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
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Cited by 13 •
2012
The compartmentalization of proteins either within the plasma membrane or into intracellular locations is one regulatory mechanism that can greatly influence signaling outcomes; hence, to understand signaling it is important to study the spatial and temporal behavior of the proteins involved. We describe here a TIRF microscopy based system to study signal transduction in T cells, but is broadly applicable.
FAQs
Although the engineered T cells share a predefined receptor, their development is still influenced by thymic selection. This process helps determine which cells mature and enter the peripheral immune system, linking receptor expression to functional T cell development. Consequently, experiments must interpret later activation or tolerance in the context of selection that occurred before the immune challenge.
Shared antigen recognition reduces variation in the receptor specificity of the cells being examined. Researchers can therefore follow clonal expansion and activation with greater experimental control than in a diverse T cell population. This uniformity is especially useful when asking how a particular peptide–MHC complex drives cellular responses or how those responses change during infection or vaccination.
The predefined receptor responds to a particular peptide–MHC complex, so antigen presentation becomes a central experimental variable. Changes in how that complex is displayed can influence T cell activation and expansion. Studying this relationship helps researchers connect presentation of pathogen- or vaccine-associated antigens with downstream cellular immunity and investigate mechanisms of immune-mediated disease.
TCR transgenic models provide a controlled population in which responses to the recognized antigen can be examined consistently. Researchers can compare whether antigen exposure is associated with expansion and functional immune responses or with tolerance, in which responsiveness is reduced or constrained. This comparison supports mechanistic analysis of how antigen recognition shapes immune outcomes.
A typical workflow begins with an organism carrying the predefined TCR genes, followed by examination of T cell development and exposure to an antigen, pathogen, or vaccine-related stimulus. Researchers then trace antigen-specific cells and measure outcomes such as clonal expansion, activation, tolerance, or memory formation. The controlled receptor specificity links these observations to a defined antigen-recognition event.
These models are valuable when investigators need to follow how antigen-specific T cells respond during infection or after vaccination. A shared receptor makes it easier to track the responding population and evaluate expansion or memory formation over the course of the study. The approach can therefore connect an antigenic stimulus with the development of cellular immunity.
After antigen-specific activation, investigators can follow the same defined T cell population to examine whether a memory response develops. Because the cells recognize a specified peptide–MHC complex, subsequent observations can be related to that antigen rather than to unknown receptor specificities. This supports controlled analysis of how immune responses persist after the initial stimulus.
Beyond infection and vaccination, these models support studies of immune-mediated disease by providing a defined population of antigen-recognizing T cells. Researchers can examine how antigen presentation and T cell activation contribute to disease-associated immune responses, while also assessing tolerance and expansion. Their controlled specificity makes mechanistic relationships easier to investigate than in an unrestricted T cell repertoire.