Axon Elongation
Axon Elongation - Related Videos
02:35
Transcription Elongation Factors
0 Views •
2020
Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene. The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA into a...
02:35
Transcription Elongation Factors
0 Views •
2023
Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene. The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA into a...
10:46
Novel Metrics to Characterize Embryonic Elongation of the Nematode Caenorhabditis elegans
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Cited by 3 •
2016
Here we detail protocols specifically designed to monitor morphogenic defects that occur during early and late phases of embryonic elongation of the nematode Caenorhabditis elegans. Ultimately, these protocols are designed to identify genes that regulate these phases and to characterize their differential requirements along the antero-posterior axis of the embryo.
10:02
Mechanical Manipulation of Neurons to Control Axonal Development
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Cited by 10 •
2011
Application and direct measurements of forces on neurons in the 2-1000 microdyne range are achieved with high precision using calibrated glass needles. This methodology can be used to control and measure several aspects of axonal development, including axonal initiation, axonal tension, velocity of axonal elongation, and force vectors.
09:25
Laser Nanosurgery of Cerebellar Axons In Vivo
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Cited by 7 •
2014
Two-photon imaging, coupled to laser nanodissection, are useful tools to study degenerative and regenerative processes in the central nervous system with subcellular resolution. This protocol shows how to label, image, and dissect single climbing fibers in the cerebellar cortex in vivo.
FAQs
The growth cone at the axon tip detects extracellular guidance cues through receptors. Receptor activation is converted into changes in actin organization and microtubule dynamics, which alter membrane protrusion and support forward movement. This coupling allows environmental information to influence the direction and progression of axon elongation during neural circuit formation.
These cytoskeletal processes provide the structural changes needed for growth cone movement. Actin organization contributes to membrane protrusion, while regulated microtubule dynamics participate in forward advance of the axon. Studying both processes helps explain how signals received at the axon tip become physical extension of the developing neuronal projection.
A permissive path provides conditions that allow the axon to extend, whereas a directed path reflects guidance information that influences where extension proceeds. The distinction emphasizes that elongation depends not only on the capacity for forward growth but also on how extracellular cues and their receptors shape the route taken by the growth cone.
Extracellular guidance cues influence axon elongation by being detected through receptors on the growth cone. Their effects are expressed through changes in actin organization and microtubule dynamics, altering protrusion and forward advance. Consequently, the local cue environment can affect whether an axon follows a permissive route or a more specifically directed path.
A useful analysis follows the relationship between extracellular guidance cues, growth-cone receptors, cytoskeletal changes, and axonal advance. Researchers can therefore connect cue detection with alterations in actin organization, microtubule dynamics, membrane protrusion, and path selection. This framework links cellular behavior at the axon tip with the larger problem of neural circuit formation.
During development, studying this process helps explain how neurons establish precise connections and form functional neural circuits. In injury research, it provides a basis for examining why damaged axons often fail to regrow in the adult nervous system. These related contexts support investigations of neural connectivity and strategies intended to promote axon regeneration.