Egfp Luciferase Expression
Egfp Luciferase Expression - Related Videos
08:09
High-throughput Titration of Luciferase-expressing Recombinant Viruses
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Cited by 14 •
2014
This article presents a high-throughput luciferase expression-based method of titrating various RNA and DNA viruses using automated and manual liquid handlers.
10:38
Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters
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Cited by 78 •
2012
Circadian clocks function within individual cells, i.e., they are cell-autonomous. Here, we describe methods for generating cell-autonomous clock models using non-invasive, luciferase-based real-time bioluminescence technology. Reporter cells provide tractable, functional model systems for studying circadian biology.
03:23
Measurement of the In Vivo Burden of Bacteria Expressing Luciferase in an Insect Larva
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2025
This video showcases the in vivo bacterial burden measurement using Bacillus Calmette-Guérin, BCG, lux-infected insect larvae. The process involves larval homogenization, treatment with a luciferase substrate, and bioluminescence measurement to determine relative light units that correlate with bacterial burden.
10:03
Development of Leishmania Species Strains with Constitutive Expression of eGFP
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2023
Here, we describe the methodology used for generating L. panamensis and L. donovani strains expressing the gene for eGFP as a stable integrated transgene using the pLEXSY system. Transfected parasites were cloned by limiting dilution, and clones with the highest fluorescence intensity in both species were selected for further use in drug screening assays.
03:17
Luciferase-tagged Glioma Stem Cell Model: A Lentivirus-mediated Method to Prepare Luciferase-tagged Glioma Stem Cells for In Vitro Therapeutic Studies
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2023
This video demonstrates the establishment of luciferase-tagged glioma stem cells via lentivirus-mediated gene delivery. Luciferase acts as a reporter gene and helps facilitate rapid drug screening experiments.
FAQs
Regulatory DNA determines when and where the reporter genes are transcribed, after which cellular translation produces the reporter molecules. This arrangement connects reporter output to promoter or transgene activity rather than treating the optical signal as an independent neuronal marker. Consequently, changes in regulatory control can be examined across cells, tissues, or living preparations.
EGFP and luciferase generate different kinds of evidence. EGFP produces green fluorescence after excitation, making labeled neurons or cellular locations visible, whereas luciferase produces bioluminescence through a substrate-dependent reaction. Their distinct signal mechanisms allow one reporter system to relate spatial localization to measurements of gene-expression activity.
Using both reporters separates two experimental questions that a single optical readout may not address equally well: where labeled cells or reporter activity is located, and how gene-expression activity changes quantitatively. In neuroscience, that pairing can connect cellular identity or localization with molecular regulation during neural development, circuit studies, or disease-related investigations.
A study uses regulatory DNA to control reporter-gene activity, then observes EGFP fluorescence to identify labeled neurons or locations and assesses luciferase bioluminescence for a complementary expression readout. The same logic can be applied across cells, tissues, or living preparations, depending on the neural material being examined.
The fluorescence channel provides visual information about labeled neurons and the distribution of reporter activity, while the luciferase channel supports measurements related to gene-expression level. Together, these outputs can associate a spatial cellular pattern with molecular changes, helping investigators compare activity across cells, tissues, or living preparations.
In neuroscience, the paired reporters can support studies of neural development, circuit function, and disease-related regulation. EGFP helps reveal which neurons or cellular regions carry the labeled activity, while luciferase contributes a complementary measure of gene-expression change. This combination is useful when researchers need both anatomical context and molecular regulation in one investigation.