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High-throughput Titration of Luciferase-expressing Recombinant Viruses
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Biology
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JoVE Journal Biology
High-throughput Titration of Luciferase-expressing Recombinant Viruses

High-throughput Titration of Luciferase-expressing Recombinant Viruses

DOI:
10.3791/51890-v

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08:09 min

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September 19, 2014

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, , , , , ,
1Center for Innovative Cancer Research,Ottawa Hospital Research Institute, 2Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine,University of Ottawa, 3Faculty of Medicine,University of Ottawa

Chapters

  • 00:05Title
  • 01:16Sample Preparation and Preparation of Permissive Cells for Virus Titration
  • 02:57Preparation of Viral Standard Curve and Transfer of Sample Supernatants on to Permissive Cells
  • 04:08Assessment of Cell Viability and Preparation of the Luciferin Substrate
  • 04:53Reading Bioluminescence and Data Analysis
  • 05:46Results: Estimated Viral Titers and Sample Cytotoxicity
  • 07:01Conclusion

Summary

Automatic Translation

Automatic Translation

This article presents a high-throughput luciferase expression-based method of titrating various RNA and DNA viruses using automated and manual liquid handlers.

Tags

Keywords: High-throughputTitrationLuciferaseRecombinant VirusesPlaque AssayInfectionLuminescenceViral TitersCytotoxicityMetabolic ViabilityDrug Screen

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