Perfused Mouse Brain
Perfused Mouse Brain - Related Videos
04:44
Antegrade Perfusion Model: An Ex Vivo Technique to Perfuse Isolated Mouse Heart
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2025
This video describes an antegrade perfusion technique of an isolated mouse heart during which a perfusate is injected through the left ventricle, and eventually, it perfuses the entire myocardium. This method can be used to isolate cardiomyocytes from murine models and generate decellularized hearts for further downstream applications.
08:19
The Mouse Isolated Perfused Kidney Technique
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Cited by 24 •
2016
The mouse isolated perfused kidney (MIPK) is a technique for keeping a mouse kidney under ex vivo conditions perfused and functional for 1 hr. The buffers and surgical technique are described in detail.
03:44
Langendorff Retrograde Heart Perfusion: An Ex Vivo Technique to Perfuse Isolated Mouse Heart
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2025
In this video, we perform Langendorff retrograde perfusion of an isolated mouse heart. During the procedure, the cardiac activity of the heart is maintained by perfusing it retrogradely with a perfusion solution via the coronary arteries using a cannula inserted into the ascending aorta. The model is used to study the cardiac pathophysiology of the heart.
27:58
Brain Slice Stimulation Using a Microfluidic Network and Standard Perfusion Chamber
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Cited by 8 •
2007
We demonstrate fabrication of a simple microfluidic device that can be integrated with standard electrophysiology setups to expose microscale surfaces of a brain slice in a well controlled manner to different neurotransmitters.
07:32
Purification of Mouse Brain Vessels
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Cited by 90 •
2015
We describe a protocol allowing the purification of the mouse brain's vascular compartment. Isolated brain vessels include endothelial cells linked by tight junctions and surrounded by a continuous basal lamina, pericytes, vascular smooth muscle cells, as well as perivascular astroglial membranes.
FAQs
Flushing blood through the cerebral vessels reduces blood-related interference before fixation and helps limit postmortem degradation. This produces tissue with more consistent structural preservation, making cellular boundaries and tissue architecture easier to examine. The improved preparation is particularly valuable for histology, microscopy, and neuroanatomical studies that depend on clear, stable anatomical detail.
The physiological solution circulates through the vascular system and helps flush blood from cerebral vessels. The fixative then stabilizes cellular and tissue architecture, preserving the brain for later analysis. Using these stages together supports more consistent tissue quality than relying on preservation after death alone, which can allow greater structural degradation.
In transcardial perfusion, fluid is introduced through the heart and carried into the cerebral blood vessels. This vascular route distributes the solution throughout the brain before fixation. Because the preparation follows the circulation system, it supports broad exposure of brain tissue and helps produce preservation suitable for examining structures across different neuroanatomical regions.
Preserved architecture supports analyses that depend on spatial relationships within the brain, including neural circuit organization, protein distribution, and disease-related structural changes. Histology, immunohistochemistry, and microscopy can use the stabilized tissue to compare cellular or molecular patterns across samples. The preparation also helps evaluate how experimental treatments affect brain structure.
The preparation begins by circulating a physiological solution through the animal’s vascular system, typically through the heart in a transcardial approach. This flushes blood from the cerebral vessels. A fixative is then circulated to stabilize the tissue. Afterward, the preserved brain can be used for histology, immunohistochemistry, microscopy, or neuroanatomical analysis.
The procedure requires a physiological solution for vascular flushing and a fixative for structural stabilization. Both are delivered through the cardiovascular route, with fluid entering the heart and passing through cerebral blood vessels. This combination links the materials to their specific roles: clearing blood first, then preserving cellular and tissue architecture for subsequent research.
Researchers choose this preparation when experiments require consistent preservation of brain structure for postmortem analysis. It is useful for studying neural circuits, protein distribution, disease-related changes, and responses to experimental treatments. The resulting tissue can support histology, immunohistochemistry, microscopy, and neuroanatomical analysis, allowing structural findings to be examined after the experimental intervention.