Surface Receptor Density
Surface Receptor Density - Related Videos
04:55
Assaying Surface Expression of Chemosensory Receptors in Heterologous Cells
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Cited by 6 •
2011
Here we demonstrate a protocol to carry out live cell staining that can be used to detect odorant receptors on the surface of HEK293T cells conveniently. In addition, it may also be used to assay for surface expression of other chemosensory receptors or GPCRs.
01:28
Types of Receptors: Cell Surface Receptors
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2023
Cell-surface receptors, also known as transmembrane receptors, are cell surface, membrane-anchored (integral) proteins that bind to external ligand molecules. This type of receptor spans the plasma membrane and performs signal transduction, converting an extracellular signal into an intracellular signal. Ligands that interact with cell-surface receptors do not have to enter the cell that they affect. Cell-surface receptors are also called cell-specific proteins or markers because they are...
08:49
Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens
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Cited by 8 •
2020
This manuscript describes a genome-scale cell-based screening approach to identify extracellular receptor-ligand interactions.
09:55
Surface Renewal: An Advanced Micrometeorological Method for Measuring and Processing Field-Scale Energy Flux Density Data
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Cited by 21 •
2013
Surface renewal is a micrometeorological method that is being used increasingly to determine energy fluxes, but its technical complexity makes it inaccessible to a broad audience. We describe the steps needed to set up and calibrate a surface renewal field station, to acquire and process data, and to correctly interpret results.
12:30
Avidity-based Extracellular Interaction Screening (AVEXIS) for the Scalable Detection of Low-affinity Extracellular Receptor-Ligand Interactions
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Cited by 56 •
2012
AVEXIS is a high throughput protein interaction assay developed to systematically screen for novel extracellular receptor-ligand pairs involved in cellular recognition processes. It is specifically designed to detect transient protein interactions that are difficult to identify using other high throughput approaches.
FAQs
Neurons can increase membrane receptor availability by inserting receptors into the plasma membrane and decrease it through endocytosis, which removes receptors from the surface. Recycling can return internalized receptors to the membrane, making density dynamic rather than fixed. The balance among these processes changes how many receptors can encounter extracellular ligands and influence downstream signaling.
Lateral movement allows receptors to redistribute within the lipid bilayer without requiring immediate insertion or removal. This can change where functional receptors are concentrated on the neuronal surface and may alter their access to extracellular signals. Examining distribution alongside total surface receptor density therefore helps distinguish changes in receptor location from changes in membrane availability.
A higher number of functional receptors at the membrane can provide more opportunities for extracellular ligands to bind, whereas receptor removal can reduce that access. Because ligand binding initiates downstream signaling, trafficking-related changes in surface abundance can modify the strength of neuronal responses. This connects membrane-level regulation with changes in cellular communication.
Receptor turnover gives neurons a way to adjust their responsiveness as signaling conditions change. Endocytosis can reduce surface availability, while recycling and new insertion can restore or increase it. These coordinated changes may support adaptation to neurotransmitters or drugs, allowing signaling strength to shift over time rather than remain determined by a static receptor population.
Measurements of surface receptor density can reveal how receptors are distributed at the membrane and how their abundance changes through turnover. Interpreted with trafficking information, these measurements help relate molecular regulation to neuronal responses. The resulting evidence can clarify how changes in receptor availability contribute to synaptic transmission, plasticity, and adaptation.
Changes in the number and distribution of functional receptors at the neuronal surface can modify how strongly neurons respond to extracellular signals. This provides a cellular link to synaptic transmission and plasticity, where altered receptor availability may accompany changes in neuronal communication. Studying density and trafficking together helps connect membrane regulation with these functional processes.
Neurotransmitters and drugs can be associated with changes in neuronal responsiveness, while disease-related signaling may also involve altered receptor regulation. Assessing surface receptor density and turnover helps identify whether signaling differences correspond to changes in membrane receptor availability. This approach links molecular changes at the cell surface with altered neuronal communication and broader circuit-related effects.